BACTERIAL COMMUNITY STRUCTURES IN SEDIMENTS OF LAKES RESPONSES TO PETROLEUM CONTAMINATION IN DAQING, CHINA

Bacterial community compositions were examined in sediments of different lakes (Lake Xidahai, Lake Qing-kenpao and Lake Longhupao) in Daqing using terminal restriction fragment length polymorphism (T-RFLP) and 16S rRNA gene clone library. The major phylogenic groups of all sediments included Proteobacteria, Firmicutes, Cyanobacteria, Actinobacteria, Nitrospira, Acidobacteria, Bacteroidetes, Chloroflexi, Planctomycetes and Verrucomicrobia among them, Proteobacteria, Acidobacteria, Bacteroidetes and Chloroflexi were the dominant phyla. The highest bacterial diversity occurred in Lake Longhupao and the lowest in Lake Qingkenpao. Relationships between environmental factors and bacterial community compositions were identified with redundancy analysis. It was identified that pH and total petroleum hydrocarbon (TPH) were related to bacterial community compositions and pH was the most influential parameter. Our results found different bacterial community compositions in sediments of three lakes, which suggested environmental factors exerted a key influence on the bacterial communities in lake ecosystems.