Single-cell Sequencing in the Field of Stem Cells

被引：15

作者：

Chen, Tian ^{[1
,2
]}

Li, Jiawei ^{[1
,2
]}

Jia, Yichen ^{[1
,2
]}

Wang, Jiyan ^{[4
]}

Sang, Ruirui ^{[5
]}

Zhang, Yi ^{[2
,3
]}

Rong, Ruiming ^{[1
,2
]}

机构：

[1] Fudan Univ, Zhongshan Hosp, Dept Urol, 180 Fenglin Rd, Shanghai 200032, Peoples R China

[2] Shanghai Key Lab Organ Transplantat, Shanghai, Peoples R China

[3] Fudan Univ, Zhongshan Hosp, Biomed Res Ctr, Inst Clin Sci, 180 Fenglin Rd, Shanghai 200032, Peoples R China

[4] Shanghai Publ Hlth Clin Ctr, Dept Urol, Shanghai, Peoples R China

[5] Fudan Univ, Zhongshan Hosp, Dept Transfus, Shanghai, Peoples R China

来源：

CURRENT GENOMICS | 2020年 / 21卷 / 08期

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

Single-cell sequencing; pluripotent stem cell; tissue-specific stem cell; cancer stem cell; RNA sequencing; heterogeneity; MESSENGER-RNA-SEQ; GENOME-WIDE; EVOLUTION; EMBRYOS; HETEROGENEITY; DIVERSITY; RENEWAL;

D O I：

10.2174/1389202921999200624154445

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Variation and heterogeneity between cells are the basic characteristics of stem cells. Traditional sequencing analysis methods often cover up this difference. Single-cell sequencing technology refers to the technology of high-throughput sequencing analysis of genomes at the single-cell level. It can effectively analyze cell heterogeneity and identify a small number of cell populations. With the continuous progress of cell sorting, nucleic acid extraction and other technologies, single-cell sequencing technology has also made great progress. Encouraging new discoveries have been made in stem cell research, including pluripotent stem cells, tissue-specific stem cells and cancer stem cells. In this review, we discuss the latest progress and future prospects of single-cell sequencing technology in the field of stem cells.

引用

页码：576 / 584

页数：9

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