YY1 represses the transcriptional activity of Runx2 in C2C12 cells

被引：8
作者：
Jeong, Hyung Min
Choi, You Hee
Lee, Sung Ho
Lee, Kwang Youl [1 ]
机构：
[1] Chonnam Natl Univ, Coll Pharm, Kwangju 500757, South Korea
来源：
MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2014年 / 383卷 / 1-2期
基金：
新加坡国家研究基金会;
关键词：
Runx2; YY1; Transcriptional activity; DNA binding affinity; BONE MORPHOGENETIC PROTEIN-2; OSTEOBLAST DIFFERENTIATION; GENE-EXPRESSION; NUCLEAR FACTOR; CBFA1; BETA; PURIFICATION; RECRUITMENT; OSF2/CBFA1; ACTIN;
D O I：
10.1016/j.mce.2013.12.001
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Runx2 is a major transcription factor that induces osteoblast differentiation by bone morphogenetic proteins (BMPs). Conversely, YY1 is a transcription factor that inhibits BMP2-induced cell differentiation. Until now, there has been no understanding of how osteoblast differentiation by Runx2 and YY1 is regulated. In this study we focused on the relationship between Runx2 and YY1. We confirmed that alkaline phosphatase staining is repressed by YY1. Runx2 interacted with YY1 through Runt and the C-terminus domain of Runx2. YY1 markedly repressed the Runx2-mediated enhancement of transcriptional activity on the osteocalcin and alkaline phosphatase promoters. Knockdown of YY1 enhanced BMP2- and Runx2-induced osteoblast differentiation. YY1 decreased Runx2 DNA binding affinity. The results indicate that YY1 represses osteoblast differentiation by an interaction with Runx2 and inhibits the transcriptional activity of Runx2. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：103 / 110
页数：8
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