Construction of an Artificial Liver Support System Based on a Reciprocating Rotating Bioreactor

被引：0

作者：

Wu, Changzhe ^{[1
]}

Huo, Xiaolin ^{[1
]}

Li, Ming ^{[1
]}

Xiu, Xiaoqing ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Inst Elect Engn, Beijing, Peoples R China

[2] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China

来源：

2010 4TH INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING (ICBBE 2010) | 2010年

关键词：

bioartificial liver; bioreactor; medicial devices; blood purification; liver failure;

D O I：

暂无

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

A novel reciprocating rotating bioreactor is proposed. Through the control of the rate and direction of the reciprocating rotation of the bioreactor as well as the direction of the fluid flow, the hepatocytes are constantly maintained in a microgravity status and suffer less shearing stress. Such a bioreactor is advantageous for the 3-dimensional growth of the hepatocytes and may hold no less than 500 grams of hepatocytes. A novel hybrid bioartificial liver support system is constructed based on the bioreactor. Oxygen is supplied efficiently to the reactor using gas dispersion and an ideal environment for the hepatocytes is created via closed-loop control of parameters of the fluid in the reactor, such as the temperature, pH, PO2 and PCO2. The system enables the function of blood purification of the non-bioartificial liver and the function as the bioartificial liver for suspension culturing of hepatocytes.

引用

页数：4

共 6 条

[1] Improving the next generation of bioartificial liver devices [J].

Allen, JW ;

Bhatia, SN .

SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 2002, 13 (06) :447-454

[2] Prospective, randomized, multicenter, controlled trial of a bioartificial liver in treating acute liver failure [J].

Demetriou, AA ;

Brown, RS ;

Busuttil, RW ;

Fair, J ;

McGuire, BM ;

Rosenthal, P ;

Esch, JSA ;

Lerut, J ;

Nyberg, SL ;

Salizzoni, M ;

Fagan, EA ;

de Hemptinne, B ;

Broelsch, CE ;

Muraca, M ;

Salmeron, JM ;

Rabkin, JM ;

Metselaar, HJ ;

Pratt, D ;

De La Mata, M ;

McChesney, LP ;

Everson, GT ;

Lavin, PT ;

Stevens, AC ;

Pitkin, Z ;

Solomon, BA .

ANNALS OF SURGERY, 2004, 239 (05) :660-667

[3]

Hui T, 2001, J Hepatobiliary Pancreat Surg, V8, P1

[4]

Jauregui HO, 1997, ARTIF ORGANS, V21, P1163

[5] Overview of extracorporeal liver support systems and clinical results [J].

McLaughlin, BE ;

Tosone, CM ;

Custer, LM ;

Mullon, C .

BIOARTIFICIAL ORGANS II: TECHNOLOGY, MEDICINE, AND MATERIALS, 1999, 875 :310-325

[6] Bioreactor cultivation conditions modulate the composition and mechanical properties of tissue-engineered cartilage [J].

Vunjak-Novakovic, G ;

Martin, I ;

Obradovic, B ;

Treppo, S ;

Grodzinsky, AJ ;

Langer, R ;

Freed, LE .

JOURNAL OF ORTHOPAEDIC RESEARCH, 1999, 17 (01) :130-138

← 1 →