Vitamin B12 offers neuronal cell protection by inhibiting Aβ-42 amyloid fibrillation

被引：101

作者：

Alam, Parvez ^{[1
]}

Siddiqi, Mohammad Khursheed ^{[1
]}

Chaturvedi, Sumit Kumar ^{[1
]}

Zaman, Masihuz ^{[1
]}

Khan, Rizwan Hasan ^{[1
]}

机构：

[1] Aligarh Muslim Univ, Mol Biophys & Biophys Chem Grp, Interdisciplinary Biotechnol Unit, Aligarh 202002, Uttar Pradesh, India

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2017年 / 99卷

关键词：

Protein aggregation; Amyloids; Neurodegenation; Cytotoxicity; ALZHEIMERS-DISEASE; PRECURSOR PROTEIN; BETA-PROTEIN; AGGREGATION; BEHAVIOR; INSIGHT; INSULIN; SURFACTANTS; MECHANISM; OLIGOMERS;

D O I：

10.1016/j.ijbiomac.2017.03.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein misfolding and aggregation has been implicated as the cause of more than 20 diseases in humans such as Alzheimer's and Parkinson's and systemic amyloidosis. Retardation of A beta-42 aggregation is considered as a promising and challenging strategy for developing effective therapeutics against Alzheimer's disease. Herein, we demonstrated the effect of vitamin B12 (VB) on inhibiting amyloid formation by employing ThT fluorescence assay, circular dichroism, ANS fluorescence assay, dynamic light scattering measurements and transmission electron microscopy and cell viability assay. Our results demonstrate that vitamin B12 (VB), inhibits A beta-42 aggregation in a concentration dependent manner. Further VB also provide protection against amyloid induced cytotoxicity in human neuronal cell line. This study points towards a promising strategy to combat A beta-42 aggregation and may have broader implication for targeting other neurological disorders whose distinct hallmark is also amyloid formation. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：477 / 482

页数：6

共 41 条

[31] Unraveling the Early Events of Amyloid-β Protein (Aβ) Aggregation: Techniques for the Determination of Aβ Aggregate Size [J].