Low-level (gallium-aluminum-arsenide) laser irradiation of Par-C10 cells and acinar cells of rat parotid gland

被引：18

作者：

Onizawa, Katsuhiro

Muramatsu, Takashi ^{[1
]}

Matsuki, Miwako ^{[1
,2
]}

Ohta, Kazumasa ^{[3
]}

Matsuzaka, Kenichi ^{[4
]}

Oda, Yutaka ^{[5
]}

Shimono, Masaki ^{[1
]}

机构：

[1] Tokyo Dent Coll, Oral Hlth Sci Ctr, Dept Pathol, Mihama Ku, Chiba, Japan

[2] Nihon Univ, Sch Dent Matsudo, Dept Physiol, Chiba, Japan

[3] Tokyo Dent Coll, Oral Hlth Sci Ctr, Dept Biochem, Mihama Ku, Chiba, Japan

[4] Tokyo Dent Coll, Oral Hlth Sci Ctr, Dept Clin Pathophysiol, Mihama Ku, Chiba, Japan

[5] Tokyo Dent Coll, Oral Hlth Sci Ctr, Dept Dent Mat Sci, Mihama Ku, Chiba, Japan

来源：

LASERS IN MEDICAL SCIENCE | 2009年 / 24卷 / 02期

关键词：

Acinar cell; Parotid gland; Low-level laser irradiation; Amylase; HSP25; LOW-ENERGY LASER; PULP CELLS; SUBMANDIBULAR GLANDS; DIODE-LASER; IN-VITRO; POWER; PROLIFERATION; REGENERATION; MICROCIRCULATION; DIFFERENTIATION;

D O I：

10.1007/s10103-008-0541-7

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

We investigated cell response, including cell proliferation and expression of heat stress protein and bcl-2, to clarify the influence of low-level [gallium-aluminum-arsenide (Ga-Al-As) diode] laser irradiation on Par-C10 cells derived from the acinar cells of rat parotid glands. Furthermore, we also investigated amylase release and cell death from irradiation in acinar cells from rat parotid glands. The number of Par-C10 cells in the laser-irradiated groups was higher than that in the non-irradiated group at days 5 and 7, and the difference was statistically significant (P < 0.01). Greater expression of heat shock protein (HSP)25 and bcl-2 was seen on days 1 and 3 in the irradiated group. Assay of the released amylase showed no significant difference statistically between the irradiated group and the non-irradiated group. Trypan blue exclusion assay revealed that there was no difference in the ratio of dead to live cells between the irradiated and the non-irradiated groups. These results suggest that low-level laser irradiation promotes cell proliferation and expression of anti-apoptosis proteins in Par-C10 cells, but it does not significantly affect amylase secretion and does not induce rapid cell death in isolated acinar cells from rat parotid glands.

引用

页码：155 / 161

页数：7

共 35 条

[1] HISTOLOGICAL STUDIES ON THE RHEUMATOID SYNOVIAL-MEMBRANE IRRADIATED WITH A LOW-ENERGY LASER [J].