Targeting Synaptic Pathology with a Novel Affinity Mass Spectrometry Approach

被引：25

作者：

Brinkmalm, Ann ^{[1
]}

Brinkmalm, Gunnar ^{[1
]}

Honer, William G. ^{[2
]}

Moreno, Julie A. ^{[3
]}

Jakobsson, Joel ^{[1
]}

Mallucci, Giovanna R. ^{[3
]}

Zetterberg, Henrik ^{[1
,4
]}

Blennow, Kaj ^{[1
]}

Oehrfelt, Annika ^{[1
]}

机构：

[1] Univ Gothenburg, Sahlgrenska Acad, Inst Neurosci & Physiol, Dept Psychiat & Neurochem, S-43180 Molndal, Sweden

[2] Univ British Columbia, Dept Psychiat, Vancouver, BC V6H 3Z6, Canada

[3] Univ Leicester, MRC Toxicol Unit, Leicester LE1 9HN, Leics, England

[4] UCL Inst Neurol, London WC1N 3BG, England

来源：

MOLECULAR & CELLULAR PROTEOMICS | 2014年 / 13卷 / 10期

基金：

加拿大健康研究院;

关键词：

FTICR-MS METHOD; ALZHEIMERS-DISEASE; DIFFERENTIAL EXPRESSION; PRESYNAPTIC PROTEINS; MEMBRANE-FUSION; HUMAN BRAIN; SNAP-25; VESICLE; ISOFORMS; COMPLEX;

D O I：

10.1074/mcp.M114.040113

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a novel strategy for studying synaptic pathology by concurrently measuring levels of four SNARE complex proteins from individual brain tissue samples. This method combines affinity purification and mass spectrometry and can be applied directly for studies of SNARE complex proteins in multiple species or modified to target other key elements in neuronal function. We use the technique to demonstrate altered levels of presynaptic proteins in Alzheimer disease patients and prion-infected mice.

引用

页码：2584 / 2592

页数：9

共 43 条

[1] Syntaxin 1A and 1B display distinct distribution patterns in the rat peripheral nervous system
Aguado, F
Majo, G
Ruiz-Montasell, B
Llorens, J
Marsal, J
Blasi, J
[J]. NEUROSCIENCE, 1999, 88 (02) : 437 - 446
[2] Barakauskas V. E., 2011, 59 ASMS C MASS SPECT
[3] A Novel Mechanism and Treatment Target for Presynaptic Abnormalities in Specific Striatal Regions in Schizophrenia
Barakauskas, Vilte E.
Beasley, Clare L.
Barr, Alasdair M.
Ypsilanti, Athena R.
Li, Hong-Ying
Thornton, Allen E.
Wong, Hubert
Rosokilja, Gorazd
Mann, J. John
Mancevski, Branislav
Jakovski, Zlatko
Davceva, Natasha
Ilievski, Boro
Dwork, Andrew J.
Falkai, Peter
Honer, William G.
[J]. NEUROPSYCHOPHARMACOLOGY, 2010, 35 (05) : 1226 - 1238
[4] Developmentally regulated switch in alternatively spliced SNAP-25 isoforms alters facilitation of synaptic transmission
Bark, C
Bellinger, FP
Kaushal, A
Mathews, JR
Partridge, LD
Wilson, MC
[J]. JOURNAL OF NEUROSCIENCE, 2004, 24 (40) : 8796 - 8805
[5] HUMAN CDNA CLONES ENCODING 2 DIFFERENT ISOFORMS OF THE NERVE-TERMINAL PROTEIN SNAP-25
BARK, IC
WILSON, MC
[J]. GENE, 1994, 139 (02) : 291 - 292
[6] DIFFERENTIAL EXPRESSION OF SNAP-25 PROTEIN ISOFORMS DURING DIVERGENT VESICLE FUSION EVENTS OF NEURAL DEVELOPMENT
BARK, IC
HAHN, KM
RYABININ, AE
WILSON, MC
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (05) : 1510 - 1514
[7] THE SYNTAXIN FAMILY OF VESICULAR TRANSPORT RECEPTORS
BENNETT, MK
GARCIAARRARAS, JE
ELFERINK, LA
PETERSON, K
FLEMING, AM
HAZUKA, CD
SCHELLER, RH
[J]. CELL, 1993, 74 (05) : 863 - 873
[8] Synaptic pathology in Alzheimer's disease: Relation to severity of dementia, but not to senile plaques, neurofibrillary tangles, or the ApoE4 allele
Blennow, K
Bogdanovic, N
Alafuzoff, I
Ekman, R
Davidsson, P
[J]. JOURNAL OF NEURAL TRANSMISSION, 1996, 103 (05) : 603 - 618
[9] NEUROPATHOLOGICAL STAGING OF ALZHEIMER-RELATED CHANGES
BRAAK, H
BRAAK, E
[J]. ACTA NEUROPATHOLOGICA, 1991, 82 (04) : 239 - 259
[10] HETEROGENEITY OF GLUTAMATERGIC AND GABAergic RELEASE MACHINERY IN CEREBRAL CORTEX: ANALYSIS OF SYNAPTOGYRIN, VESICLE-ASSOCIATED MEMBRANE PROTEIN, AND SYNTAXIN
Bragina, L.
Giovedi, S.
Barbaresi, P.
Benfenati, F.
Conti, F.
[J]. NEUROSCIENCE, 2010, 165 (03) : 934 - 943

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