A new rhodamine derived fluorescent sensor: Detection of Hg2+ at cellular level

被引:17
作者
Kuchlyan, Jagannath [1 ]
Basak, Shyam [1 ]
Dutta, Debabrata [2 ]
Das, Amit Kumar [2 ]
Mal, Dipakranjan [1 ]
Sarkar, Nilmoni [1 ]
机构
[1] Indian Inst Technol, Dept Chem, Kharagpur 721302, W Bengal, India
[2] Indian Inst Technol, Dept Biotechnol, Kharagpur 721302, W Bengal, India
关键词
Fluorescence correlation spectroscopy; Fluorescence lifetime imaging microscopy; Cell culture; Cytotoxicity; SELECTIVE DETECTION; MERCURY LEVELS; TURN-ON; CHEMOSENSOR; SPECTROSCOPY; RECOGNITION; DERIVATIVES; EXPOSURE; REMOVAL; HEALTH;
D O I
10.1016/j.cplett.2017.02.003
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Mercury ion is of great threat to the human kind due to its high toxicity in live systems. Consequently, its detection at nanolevel is of current interest. The rhodamine derivative is one of the rarest examples of fluorescent chemosensors for Hg2+, wherein a phthalaldehydic acid moiety which shows antibacterial activity, is responsible for specific binding. By using fluorescence correlation spectroscopic (FCS) study of the probe, it has been possible to detect Hg2+ in solution at a level as low as 5 pM. This sensing, applicable to fluorescence lifetime imaging of live cells, provides a novel microscopic technique. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码:84 / 88
页数:5
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