Direct organogenesis and somatic embryogenesis in Beloperone plumbaginifolia (Jacq.) Nees

Beloperone plumbaginifolia (Jacq.) Nees is a small branched medicinal shrub. In vitro studies were conducted, employing explants from node, intermode, petiole, shoot bud and leaf lamina. Murashige and Skoog (MS) medium fortified with 6.66 mu M BA enabled the proliferation of axillary and apical buds. MS medium supplemented with 5.37 mu m NAA was better for callogenesis from nodal and internodal explants, while a combination of LBA (2.46 mu M) and 2,4-D (4.52 mu M was good for leaf lamina explant. MS medium with 5.37 mu M NAA and 2.22 mu M BA was found superior for shoot induction from nodal explants. Half-strength MS medium with 5.37 mu M NAA induced adventitious roots and 85% plantlets survived when transferred in the field conditions.