Synthetic and genetically encoded fluorescent neural activity indicators

被引:33
作者
Deo, Claire [1 ,2 ]
Lavis, Luke D. [1 ,2 ]
机构
[1] Janelia Res Campus, Howard Hughes Med Inst, 19700 Helix Dr, Ashburn, VA 20147 USA
[2] Janelia Res Campus, Howard Hughes Med Inst, 19700 Helix Dr, Ashburn, VA 20147 USA
关键词
PROTEIN VOLTAGE; CALCIUM INDICATORS; ACTION-POTENTIALS; MEMBRANE-BINDING; SENSOR PROTEINS; IN-VIVO; NEURONS; MOLECULES; PROBE; EXOCYTOSIS;
D O I
10.1016/j.conb.2018.01.003
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The ultimate goal of neuroscience is to relate the complex activity of cells and cell-networks to behavior and cognition. This requires tools and techniques to visualize neuronal activity. Fluorescence microscopy is an ideal tool to measure activity of cells in the brain due to the high sensitivity of the technique and the growing portfolio of optical hardware and fluorescent sensors. Here, we give a chemist's perspective on the recent progress of fluorescent activity indicators that enable the measurement of cellular events in the living brain. We discuss advances in both chemical and genetically encoded sensors and look forward to hybrid indicators, which incorporate synthetic organic dyes into genetically encoded protein constructs.
引用
收藏
页码:101 / 108
页数:8
相关论文
共 61 条
[1]   A Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices [J].
Abdelfattah, Ahmed S. ;
Farhi, Samouil L. ;
Zhao, Yongxin ;
Brinks, Daan ;
Zou, Peng ;
Ruangkittisakul, Araya ;
Platisa, Jelena ;
Pieribone, Vincent A. ;
Ballanyi, Klaus ;
Cohen, Adam E. ;
Campbell, Robert E. .
JOURNAL OF NEUROSCIENCE, 2016, 36 (08) :2458-2472
[2]   FLUORESCENCE RATIO IMAGING OF CYCLIC-AMP IN SINGLE CELLS [J].
ADAMS, SR ;
HAROOTUNIAN, AT ;
BUECHLER, YJ ;
TAYLOR, SS ;
TSIEN, RY .
NATURE, 1991, 349 (6311) :694-697
[3]  
Berlin S, 2015, NAT METHODS, V12, P852, DOI [10.1038/NMETH.3480, 10.1038/nmeth.3480]
[4]   Recent developments of genetically encoded optical sensors for cell biology [J].
Bolbat, Andrey ;
Schultz, Carsten .
BIOLOGY OF THE CELL, 2017, 109 (01) :1-+
[5]  
Brenner S., 2002, GENOME BIOL, V3
[6]   A Semisynthetic Fluorescent Sensor Protein for Glutamate [J].
Brun, Matthias A. ;
Tan, Kui-Thong ;
Griss, Rudolf ;
Kielkowska, Anna ;
Reymond, Luc ;
Johnsson, Kai .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2012, 134 (18) :7676-7678
[7]   Semisynthetic Fluorescent Sensor Proteins Based on Self-Labeling Protein Tags [J].
Brun, Matthias A. ;
Tan, Kui-Thong ;
Nakata, Eiji ;
Hinner, Marlon J. ;
Johnsson, Kai .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (16) :5873-5884
[8]   Ultrasensitive fluorescent proteins for imaging neuronal activity [J].
Chen, Tsai-Wen ;
Wardill, Trevor J. ;
Sun, Yi ;
Pulver, Stefan R. ;
Renninger, Sabine L. ;
Baohan, Amy ;
Schreiter, Eric R. ;
Kerr, Rex A. ;
Orger, Michael B. ;
Jayaraman, Vivek ;
Looger, Loren L. ;
Svoboda, Karel ;
Kim, Douglas S. .
NATURE, 2013, 499 (7458) :295-+
[9]   Ca-NIR: a ratiometric near-infrared calcium probe based on a dihydroxanthene-hemicyanine fluorophore [J].
Collot, Mayeul ;
Ponsot, Flavien ;
Klymchenko, Andrey S. .
CHEMICAL COMMUNICATIONS, 2017, 53 (45) :6117-6120
[10]   CaRuby-Nano: a novel high affinity calcium probe for dual color imaging [J].
Collot, Mayeul ;
Wilms, Christian D. ;
Bentkhayet, Asma ;
Marcaggi, Paikan ;
Couchman, Kiri ;
Charpak, Serge ;
Dieudonne, Stephane ;
Haeusser, Michael ;
Feltz, Anne ;
Mallet, Jean-Maurice .
ELIFE, 2015, 4