A ratiometric electrochemiluminescence resonance energy transfer platform based on novel dye BODIPY derivatives for sensitive detection of lactoferrin

被引：33

作者：

Lu, Yao ^{[1
]}

Ke, Hong ^{[1
]}

Wang, Yang ^{[1
]}

Zhang, Yao ^{[1
]}

Li, Huan ^{[1
]}

Huang, Chusen ^{[1
]}

Jia, Nengqin ^{[1
]}

机构：

[1] Shanghai Normal Univ, Shanghai Key Lab Rare Earth Funct Mat, Coll Chem & Mat Sci, Educ Minist,Key Lab Resource Chem, Shanghai 200234, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2020年 / 170卷

关键词：

Ratiometric electrochemiluminescence resonance energy transfer; BODIPY derivative; Lactoferrin; DRY EYE DISEASE; ELECTROGENERATED CHEMILUMINESCENCE; QUANTUM DOTS; ELECTROCHEMISTRY; DIAGNOSIS; SYMPTOMS; SYSTEM; SIGNS; MILK;

D O I：

10.1016/j.bios.2020.112664

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A ratiometric electrochemiluminescence resonance energy transfer (ECL-RET) platform depended on novel dye BODIPY derivatives was proposed for rapid detection of lactoferrin. This ECL-RET platform is composed of aptamer decorated BODIPY composites and C-60@BSA, in which BODIPY derivative is the ECL probe and can generate significant resonance energy transfer with K2S2O8. BODIPY derivative and K2S2O8 are used as built-in reference signal and calibration signal respectively to eliminate background signal and abnormal change signal by double signal self-calibration process. At the same time, C-60, as the accelerator of K2S2O8, can effectively increase the ECL signal and further transfer as much energy as possible to BODIPY derivative. Under optimal conditions, the constructed ECL-RET platform exhibited sensitive detection of lactoferrin in the wide linear range of 10-4850 ng/mL with a LOD of 42 fg/mL. Meanwhile, the proposed ECL-RET aptasensor demonstrated superior stability, specificity and reproducibility, displaying favorable application value in practical diagnosis of this method.

引用

页数：8

共 44 条

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