Digital quantification using amplified single-molecule detection

被引：163

作者：

Jarvius, Jonas

Melin, Jonas

Goransson, Jenny

Stenberg, Johan

Fredriksson, Simon

Gonzalez-Rey, Carlos

Bertilsson, Stefan

Nilsson, Mats ^{[1
]}

机构：

[1] Univ Uppsala, Dept Genet & Pathol, Rudbeck Lab, S-75185 Uppsala, Sweden

[2] Univ Uppsala, Angstron Lab, Dept Engn Sci, S-75121 Uppsala, Sweden

[3] Univ Uppsala, BMC, Limnol Dept Ecol & Evolut, S-75123 Uppsala, Sweden

来源：

NATURE METHODS | 2006年 / 3卷 / 09期

关键词：

D O I：

10.1038/NMETH916

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a scheme for biomolecule enumeration by converting nanometer-scale specific molecular recognition events mediated by rolling-circle amplification to fluorescent micrometer-sized DNA molecules amenable to discrete optical detection. Our amplified single-molecule detection (SMD) approach preserves the discrete nature of the molecular population, allowing multiplex detection and highly precise quantification of molecules over a dynamic range of seven orders of magnitude. We apply the method for sensitive detection and quantification of the bacterial pathogen Vibrio cholerae.

引用

收藏

页码：725 / 727

页数：3

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