Exosomal lncRNA HEIH promotes cisplatin resistance in tongue squamous cell carcinoma via targeting miR-3619-5p/HDGF axis

被引:20
作者
Wang, Xiaowei [1 ]
Yu, Huiming [2 ]
Yu, Zhifen [3 ]
Wang, Dazhao [2 ]
机构
[1] Nanjing Med Univ, Dept Oncol, Affiliated Huaian Peoples Hosp 1, Huaian 223300, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Dept Stomatol, Affiliated Huaian Peoples Hosp 1, Huaian 223300, Jiangsu, Peoples R China
[3] Huaian Maternal & Child Hlth Hosp, Dept Stomatol, Huaian 223300, Jiangsu, Peoples R China
关键词
HEIH; miR-3619-5p; HDGF; TSCC; Exosomes; GASTRIC-CANCER; TUMORIGENESIS;
D O I
10.1016/j.acthis.2020.151647
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Background: Accumulating evidence has suggested that long noncoding RNAs (lncRNAs) are involved in the progression of types of human cancers. It has been known that exosomes can mediate cell-cell crosstalk by transferring lncRNAs in tumor progression. This study aimed to investigate the role of exosomal lncRNA HEIH on cisplatin (DDP) resistance in tongue squamous cell carcinoma (TSCC). Methods: The expression of HEIH in human oral keratinocytes cell line (HOK), DDP-sensitive TSCC cell line (SCC4/S) and DDP-resistant TSCC cell line (SCC4/DDP) was measured. SCC4/S and SCC4/DDP cells were transfected with sh-HEIH to examine TSCC cell proliferation and apoptosis. The DDP-resistant exosomes were extracted and identified. The expression of miR-3619-5p and TDGF in DDP-sensitive recipient cells was determined. The binding capacity between HEIH and miR-3619-5p, along with miR-3619-5p and TDGF was verified. Results: HEIH expression was significantly upregulated in SCC4/DDP cells. Downregulation of HEIH inhibited DDP resistance and cell proliferation and promoted cell apoptosis. HEIH acted as a competing endogenous RNA (ceRNA) for miR-3619-5p to upregulate HDGF expression. Exosomal HEIH promoted cell proliferation and drug resistance and inhibited cell apoptosis by sponging miR-3169-5p and upregulating HDGF. Conclusion: Exosomal HEIH acted as a ceRNA for miR-3619-5p to upregulate HDGF, thereby promoting DDP resistance in TSCC cells.
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页数:9
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