Helicobacter pylori-infected C57BL/6 mice with different gastrointestinal microbiota have contrasting gastric pathology, microbial and host immune responses

被引：26

作者：

Ge, Zhongming ^{[1
]}

Sheh, Alexander ^{[1
]}

Feng, Yan ^{[1
]}

Muthupalani, Sureshkumar ^{[1
]}

Ge, Lili ^{[1
]}

Wang, Chuanwu ^{[1
]}

Kurnick, Susanna ^{[1
]}

Mannion, Anthony ^{[1
]}

Whary, Mark T. ^{[1
]}

Fox, James G. ^{[1
]}

机构：

[1] MIT, Div Comparat Med, Cambridge, MA 02139 USA

来源：

SCIENTIFIC REPORTS | 2018年 / 8卷

关键词：

SEGMENTED FILAMENTOUS BACTERIA; CELLS; COLONIZATION; CANCER; INFLAMMATION; POPULATIONS; DEVELOP; MUCOSA; HEALTH; IGA;

D O I：

10.1038/s41598-018-25927-2

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

C57BL/6 (B6) mice from Taconic Sciences (Tac) and the Jackson Laboratory (Jax) were infected with H. pylori PMSS1 (Hp) for 16 week; there was no significant difference in the gastric histologic activity index between Hp infected Tac and Jax B6. However, the degree of gastric mucous metaplasia and Th1-associated IgG2c levels in response to Hp infection were increased in Tac mice over Jax mice, whereas the colonization levels of gastric Hp were higher by 8-fold in Jax B6 compared with Tac B6. Additionally, mRNA expression of gastric Il-1 beta, Il-17A and RegIII gamma were significantly lower in the infected Tac compared to the infected Jax mice. There were significant differences in the microbial community structures in stomach, colon, and feces between Jax and Tac B6 females. Differences in gastric microbial communities between Jax and Tac B6 females are predicted to affect the metagenome. Moreover, Hp infection perturbed the microbial community structures in the stomach, colon and feces of Jax mice, but only altered the colonic microbial composition of Tac mice. Our data indicate that the GI microbiome of Tac B6 mice is compositionally distinct from Jax B6 mice, which likely resulted in different pathological, immunological, and microbial responses to Hp infection.

引用

页数：15

共 56 条

[1] Gastrointestinal Malignancy and the Microbiome [J].