DNA-based biosensors with external Nafion and chitosan membranes for the evaluation of the antioxidant activity of beer, coffee, and tea

被引：33

作者：

Hlavata, Lenka ^{[1
]}

Vyskocil, Vlastimil ^{[2
]}

Benikova, Katarina ^{[1
]}

Borbelyova, Monika ^{[1
]}

Labuda, Jan ^{[1
]}

机构：

[1] Slovak Univ Technol Bratislava, Fac Chem & Food Technol, Inst Analyt Chem, Bratislava 81237, Slovakia

[2] Charles Univ Prague, Univ Res Ctr UNCE Supramol Chem, UNESCO Lab Environm Electrochem, Dept Analyt Chem,Fac Sci, Prague 12843 2, Czech Republic

来源：

CENTRAL EUROPEAN JOURNAL OF CHEMISTRY | 2014年 / 12卷 / 05期

关键词：

Electrochemical biosensors; Outer-sphere protective membranes; DNA damage; Reactive oxygen species; Antioxidant properties of beverages; CAPACITY; DAMAGE; CHITIN; TOOL;

D O I：

10.2478/s11532-014-0516-4

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Novel electrochemical DNA-based biosensors with outer-sphere Nafion and chitosan protective membranes were prepared for the evaluation of antioxidant properties of beverages (beer, coffee, and black tea) against prooxidant hydroxyl radicals. A carbon working electrode of a screen-printed three-electrode assembly was modified using a layer-by-layer deposition technique with low molecular weight double-stranded DNA and a Nafion or chitosan film. The membrane-covered DNA biosensors were initially tested with respect to their voltammetric and impedimetric response after the incubation of the beverage and the medium exchange for the solution of the redox indicator [Fe(CN)(6)](3-/4-). While the Nafion-protected biosensor proved to be suitable for beer and black tea extracts, the chitosan-protected biosensor was successfully used in a coffee extract. Afterwards, the applicability was successfully verified for these biosensors for the detection of a deep degradation of the surface-attached DNA at the incubation in the cleavage agent (hydroxyl radicals generated via Fenton reaction) and for the evaluation of antioxidant properties of coffee and black tea extracts against prooxidant hydroxyl radicals. The investigation of the novel biosensors with a protective membrane represents a significant contribution to the field of electrochemical DNA biosensors utilization.

引用

页码：604 / 611

页数：8

共 31 条

[1] Methods of measurement and evaluation of natural antioxidant capacity/activity (IUPAC Technical Report) [J].

Apak, Resat ;

Gorinstein, Shela ;

Bohm, Volker ;

Schaich, Karen M. ;

Ozyurek, Mustafa ;

Guclu, Kubilay .

PURE AND APPLIED CHEMISTRY, 2013, 85 (05) :957-998

[2] ATM deficiency and oxidative stress: a new dimension of defective response to DNA damage [J].

Barzilai, A ;

Rotman, G ;

Shiloh, Y .

DNA REPAIR, 2002, 1 (01) :3-25

[3] Preparation, Proton Conductivity and Mechanical Properties of Nafion 117-Zirconium Phosphate Sulphophenylphosphonate Composite Membranes [J].

Casciola, M. ;

Capitani, D. ;

Donnadio, A. ;

Frittella, V. ;

Pica, M. ;

Sganappa, M. .

FUEL CELLS, 2009, 9 (04) :381-386

[4]

de Alvarenga ES, 2011, BIOTECHNOLOGY OF BIOPOLYMERS, P91

[5] Anti/pro-oxidative properties of selected standard chemicals and tea extracts investigated by DNA-based electrochemical biosensor [J].

Ferancová, A ;

Heilerová, L ;

Korgová, E ;

Silhár, S ;

Stepánek, I ;

Labuda, J .

EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2004, 219 (04) :416-420

[6]

Fojta M, 2005, PERSP BIOANAL, V1, P385, DOI 10.1016/S1871-0069(05)01012-8

[7] A subset of ATM- and ATR-dependent phosphorylation events requires the BRCA1 protein [J].

Foray, N ;

Marot, D ;

Gabriel, A ;

Randrianarison, V ;

Carr, AM ;

Perricaudet, M ;

Ashworth, A ;

Jeggo, P .

EMBO JOURNAL, 2003, 22 (11) :2860-2871

[8] Polymer interfaces used in electrochemical DNA-based biosensors [J].

Galandova, Julia ;

Labuda, Jan .

CHEMICAL PAPERS, 2009, 63 (01) :1-14

[9]

HEILEROVA L, 2003, J FOOD SCI, V21, P78

[10]

Kumar MNVR, 2000, REACT FUNCT POLYM, V46, P1

← 1 2 3 4 →