Cholesterol-Regulated Stress Fiber Formation

被引:29
作者
Qi, Maosong [1 ]
Liu, Yuzhen [1 ]
Freeman, Michael R. [2 ,3 ,4 ]
Solomn, Keith R. [1 ,2 ,5 ]
机构
[1] Harvard Univ, Sch Med, Dept Orthopaed Surg, Boston, MA 02115 USA
[2] Harvard Univ, Sch Med, Childrens Hosp, Dept Urol, Boston, MA 02115 USA
[3] Harvard Univ, Sch Med, Dept Surg, Boston, MA 02115 USA
[4] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA
[5] Harvard Univ, Sch Med, Dept Orthopaed Surg, Boston, MA 02115 USA
基金
美国国家卫生研究院;
关键词
CHOLESTEROL; STRESS FIBER; RHO; ROCK; SRC; CAVEOLIN-1; SRC TYROSINE KINASES; SMOOTH-MUSCLE-CELLS; PLASMA-MEMBRANE; LIPID RAFTS; ACTIN CYTOSKELETON; C-SRC; CELLULAR CHOLESTEROL; OXIDATIVE-STRESS; FOCAL ADHESIONS; IN-VITRO;
D O I
10.1002/jcb.22081
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Dynamic interactions between cellular membranes and the cytoskeleton are known to play major roles in many cellular responses to environmental cues. External signals resulting in proliferation, differentiation, polarization, and motility must be translated from chemical signals into changes of state, often involving the cytoskeleton-dependent altering of cell shape and redistribution of molecules. Cholesterol, a critical component of eukaryotic cell membranes, performs vital roles in regulating membrane dynamics and function. Here we demonstrate, using mesenchymal and epithelial cell lines, that depletion of membrane cholesterol results in Src kinase-mediated Rho activation and caveolin phosphorylation, which together collaborate to form stress fibers. These results demonstrate that cholesterol is a critical regulator of membrane-cytoskeletal dynamics and suggest that altered cholesterol concentrations may result in dramatic changes in cellular responses mediated by the cytoskeleton. J. Cell. Biochem. 106: 1031-1040, 2009. (c) 2009 Wiley-Liss, Inc.
引用
收藏
页码:1031 / 1040
页数:10
相关论文
共 66 条
[1]   Regulation of integrin growth factor interactions in oligodendrocytes by lipid raft microdomains [J].
Baron, W ;
Decker, L ;
Colognato, H ;
ffrench-Constant, C .
CURRENT BIOLOGY, 2003, 13 (02) :151-155
[2]   Loss of caveolin-1 polarity impedes endothelial cell polarization and directional movement [J].
Beardsley, A ;
Fang, K ;
Mertz, H ;
Castranova, V ;
Friend, S ;
Liu, J .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (05) :3541-3547
[3]   Remodeling of the actin cytoskeleton during mammalian sperm capacitation and acrosome reaction [J].
Brener, E ;
Rubinstein, S ;
Cohen, G ;
Shternall, K ;
Rivlin, J ;
Breitbart, H .
BIOLOGY OF REPRODUCTION, 2003, 68 (03) :837-845
[4]  
BURRIDGE K, 1986, Cancer Reviews, V4, P18
[5]   Cholesterol depletion increases membrane stiffness of aortic endothelial cells [J].
Byfield, FJ ;
Aranda-Espinoza, H ;
Romanenko, VG ;
Rothblat, GH ;
Levitan, I .
BIOPHYSICAL JOURNAL, 2004, 87 (05) :3336-3343
[6]   C-SRC REGULATES THE SIMULTANEOUS REARRANGEMENT OF ACTIN CYTOSKELETON, P190RHOGAP, AND P120RASGAP FOLLOWING EPIDERMAL GROWTH-FACTOR STIMULATION [J].
CHANG, JH ;
GILL, S ;
SETTLEMAN, J ;
PARSONS, SJ .
JOURNAL OF CELL BIOLOGY, 1995, 130 (02) :355-368
[7]   CAVEOLIN CYCLES BETWEEN PLASMA-MEMBRANE CAVEOLAE AND THE GOLGI-COMPLEX BY MICROTUBULE-DEPENDENT AND MICROTUBULE-INDEPENDENT STEPS [J].
CONRAD, PA ;
SMART, EJ ;
YING, YS ;
ANDERSON, RGW ;
BLOOM, GS .
JOURNAL OF CELL BIOLOGY, 1995, 131 (06) :1421-1433
[8]   Integrins regulate Rac targeting by internalization of membrane domains [J].
del Pozo, MA ;
Alderson, NB ;
Kiosses, WB ;
Chiang, HH ;
Anderson, RGW ;
Schwartz, MA .
SCIENCE, 2004, 303 (5659) :839-842
[9]  
DVORNIK D, 1968, J LIPID RES, V9, P587
[10]   Caveolin-1α and -1β perform nonredundant roles in early vertebrate development [J].
Fang, Ping-Ke ;
Solomon, Keith R. ;
Zhuang, Liyan ;
Qi, Maosong ;
McKee, Mary ;
Freeman, Michael R. ;
Yelick, Pamela C. .
AMERICAN JOURNAL OF PATHOLOGY, 2006, 169 (06) :2209-2222