miR-140-5p suppresses BMP2-mediated osteogenesis in undifferentiated human mesenchymal stem cells

被引：144

作者：

Hwang, Supyong ^{[1
]}

Park, Seul-Ki ^{[1
,2
]}

Lee, Ha Yeon ^{[1
]}

Kim, Seong Who ^{[3
]}

Lee, Jung Shin ^{[4
,5
]}

Choi, Eun Kyung ^{[4
,6
,7
]}

You, Dalsan ^{[8
]}

Kim, Choung-Soo ^{[8
]}

Suh, Nayoung ^{[1
,2
]}

机构：

[1] Asan Med Ctr, Asan Inst Life Sci, Seoul 138736, South Korea

[2] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Med, Seoul 138736, South Korea

[3] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Biochem & Mol Biol, Seoul 138736, South Korea

[4] Univ Ulsan, Coll Med, Asan Med Ctr, Inst Innovat Canc Res, Seoul 138736, South Korea

[5] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Internal Med, Seoul 138736, South Korea

[6] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Radiat Oncol, Seoul 138736, South Korea

[7] Univ Ulsan, Coll Med, Asan Med Ctr, Ctr Dev & Commercializat Anticanc Therapeut, Seoul 138736, South Korea

[8] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Urol, Seoul 138736, South Korea

来源：

FEBS LETTERS | 2014年 / 588卷 / 17期

基金：

新加坡国家研究基金会;

关键词：

Human mesenchymal stem cells; miR-140-5p; Bone morphogenic protein 2; Differentiation; Osteogenic lineage commitment; HUMAN ADIPOSE-TISSUE; OSTEOBLAST DIFFERENTIATION; MICRORNA SIGNATURE; BONE-FORMATION; TARGETS; PROLIFERATION; BIOGENESIS; LINEAGE; MARROW; BETA;

D O I：

10.1016/j.febslet.2014.05.048

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human mesenchymal stem cells (hMSCs) have self-renewal and differentiation capabilities but the regulatory mechanisms of MSC fate determination remain poorly understood. Here, we aimed to identify microRNAs enriched in hMSCs that modulate differentiation commitments. Microarray analysis revealed that miR-140-5p is commonly enriched in undifferentiated hMSCs from various tissue sources. Moreover, bioinformatic analysis and luciferase reporter assay validated that miR-140-5p directly represses bone morphogenic protein 2 (BMP2). Furthermore, blocking miR-140-5p in hMSCs increased the expression of BMP signaling components and critical regulators of osteogenic differentiation. We propose that miR-140-5p functionally inhibits osteogenic lineage commitment in undifferentiated hMSCs. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2957 / 2963

页数：7

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