Automated three-dimensional tracking of living cells by digital holographic microscopy

被引：130

作者：

Langehanenberg, Patrik ^{[1
]}

Ivanova, Lyubomira ^{[2
]}

Bernhardt, Ingolf ^{[2
]}

Ketelhut, Steffi ^{[1
]}

Vollmer, Angelika ^{[1
]}

Dirksen, Dieter ^{[3
]}

Georgiev, Georgi ^{[4
]}

von Bally, Gert ^{[1
]}

Kemper, Bjoern ^{[1
]}

机构：

[1] Univ Med Ctr Muenster, Ctr Biomed Opt & Photon, D-48149 Munster, Germany

[2] Univ Saarland, Biophys Lab, Fac Nat & Tech Sci 3, D-66041 Saarbrucken, Germany

[3] Univ Med Ctr Muenster, Dept Prosthodont, D-48149 Munster, Germany

[4] St Klement Ohridski Univ Sofia, Dept Organ Chem Technol, Fac Chem, Sofia 1164, Bulgaria

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2009年 / 14卷 / 01期

关键词：

digital holographic microscopy; automated three-dimensional cell tracking; live cell imaging; PHASE-CONTRAST MICROSCOPY; NUMERICAL RECONSTRUCTION; REFRACTIVE-INDEX; SEDIMENTATION; MORPHOMETRY; DYNAMICS;

D O I：

10.1117/1.3080133

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Digital holographic microscopy (DHM) enables a quantitative multifocus phase contrast imaging that has been found suitable for technical inspection and quantitative live cell imaging. The combination of DHM with fast and robust autofocus algorithms enables subsequent automated focus realignment by numerical propagation of the digital holographically reconstructed object wave. In combination with a calibrated optical imaging system, the obtained propagation data quantify axial displacements of the investigated sample. The evaluation of quantitative DHM phase contrast images also enables an effective determination of lateral cell displacements. Thus, 3-D displacement data are provided. Results from investigations on sedimenting red blood cells and HT-1080 fibrosarcoma cells in a collagen tissue model demonstrate that DHM enables marker-free automated quantitative dynamic 3-D cell tracking without mechanical focus adjustment. (c) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3080133]

引用

页数：7

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