Granada Crystallisation Box:: a new device for protein crystallisation by counter-diffusion techniques

被引：88

作者：

Garcia-Ruiz, JM ^{[1
]}

Gonzalez-Ramirez, LA ^{[1
]}

Gavira, JA ^{[1
]}

Otálora, F ^{[1
]}

机构：

[1] Univ Granada, Fac Ciencias, IACT, Lab Estudios Cristalog, Granada 18002, Spain

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2002年 / 58卷

关键词：

crystallisation device; protein; counter-diffusion;

D O I：

10.1107/S0907444902014464

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Granada Crystallisation Box (GCB) is a new crystallisation device designed to perform counter-diffusion experiments. Here we describe the device and its use for protein crystallisation purposes. GCB allows one to explore and exploit the coupling between crystallisation and diffusion. The role of viscous fluids, gels and/or microgravity can be enhanced by using capillary volumes, creating a perfect diffusive mass transport scenario. The use of capillaries also reduces the consumption of macromolecules and avoids the handling of crystals for X-ray diffraction data collection.

引用

页码：1638 / 1642

页数：5

共 14 条

[1]

DUCRUIX A, 1999, CRYSTALLISATION NUCL

[2] Lysozyme crystal growth, as observed by small angle X-ray scattering, proceeds without crystallization intermediates [J].

Finet, S ;

Bonneté, F ;

Frouin, J ;

Provost, K ;

Tardieu, A .

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 1998, 27 (03) :263-271

[3] Reinforced protein crystals [J].

Garcia-Ruiz, JM ;

Gavira, JA ;

Otalora, F ;

Guasch, A ;

Coll, M .

MATERIALS RESEARCH BULLETIN, 1998, 33 (11) :1593-1598

[4] CRYSTAL QUALITY OF LYSOZYME SINGLE-CRYSTALS GROWN BY THE GEL ACUPUNCTURE METHOD [J].

GARCIARUIZ, JM ;

MORENO, A ;

VIEDMA, C ;

COLL, M .

MATERIALS RESEARCH BULLETIN, 1993, 28 (06) :541-546

[5]

GARCIARUIZ JM, 1991, CRYSTALLINE MATERIALS : GROWTH AND CHARACTERIZATION, P87, DOI 10.4028/www.scientific.net/KEM.58.87

[6]

GARCIARUIZ JM, 2002, IN PRESS METHODS ENZ

[7] Ab initio crystallographic structure determination of insulin from protein to electron density without crystal handling [J].

Gavira, JA ;

Toh, D ;

Lopéz-Jaramillo, J ;

García-Ruíz, JM ;

Ng, JD .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2002, 58 :1147-1154

[8] CRYSTAL-GROWTH IN GELS AND LIESEGANG RING FORMATION .1. DIFFUSION RELATIONSHIPS [J].

HENISCH, HK ;

GARCIARUIZ, JM .

JOURNAL OF CRYSTAL GROWTH, 1986, 75 (02) :195-202

[9] Crystallization and cryocrystallography inside X-ray capillaries [J].

López-Jaramillo, FJ ;

García-Ruiz, JM ;

Gavira, JA ;

Otálora, F .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 2001, 34 :365-370

[10]

MCPHERSON A, 1999, CRYSTALLISATION BIOL

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