Polydopamine-Functionalization of Graphene Oxide to Enable Dual Signal Amplification for Sensitive Surface Plasmon Resonance Imaging Detection of Biomarker

被引：129

作者：

Hu, Weihua ^{[1
,2
,3
,5
]}

He, Guangli ^{[1
,2
,3
]}

Zhang, Huanhuan ^{[1
,2
,3
]}

Wu, Xiaoshuai ^{[1
,2
,3
]}

Li, Jialin ^{[1
,2
,3
]}

Zhao, Zhiliang ^{[1
,2
,3
]}

Qiao, Yan ^{[1
,2
,3
]}

Lu, Zhisong ^{[1
,2
,3
]}

Liu, Yang ^{[4
]}

Li, Chang Ming ^{[1
,2
,3
]}

机构：

[1] Southwest Univ, Inst Clean Energy & Adv Mat, Chongqing 400715, Peoples R China

[2] Southwest Univ, Chongqing Key Lab Adv Mat & Technol Clean Energie, Chongqing 400715, Peoples R China

[3] Southwest Univ, Fac Mat & Energy, Chongqing 400715, Peoples R China

[4] Chinese Acad Agr Sci, Key Lab Agroprod Proc, Inst Agroprod Proc Sci & Technol, Minist Agr, Beijing 100193, Peoples R China

[5] Wuhan Univ, Minist Educ, Key Lab Analyt Chem Biol & Med, Wuhan 430072, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2014年 / 86卷 / 09期

基金：

中国国家自然科学基金;

关键词：

CARCINOEMBRYONIC ANTIGEN CEA; MICROARRAYS; BIOSENSOR; POLYMERIZATION; IMMUNOASSAY; DOPAMINE; CONTRAST; DESIGN; MARKER;

D O I：

10.1021/ac5003905

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Surface plasmon resonance imaging (SPRi) is one of the powerful tools for immunoassays with advantages of label-free, real-time, and high-throughput; however, it often suffers from limited sensitivity. Herein we report a dual signal amplification strategy utilizing polydopamine (PDA) functionalization of reduced graphene oxide (PDA-rGO) nanosheets for sensitive SPRi immunoassay in serum. The PDA-rGO nanosheet is synthesized by oxidative polymerization of dopamine in a gentle alkaline solution in the presence of graphene oxide (GO) sheets and then is antibody-conjugated via a spontaneous reaction between the protein and the PDA component. In the dual amplification mode, the first signal comes from capture of the antibody-conjugated PDA-rGO to form sandwiched immunocomplexes on the SPRi chip, followed by a PDA-induced spontaneous gold reductive deposition on PDA-rGO to further enhance the SPRi signal. The detection limit as low as 500 pg mL(-1) is achieved on a nonfouling SPRi chip with high specificity and a wide dynamic range for a model biomarker, carcinoembryonic antigen (CEA) in 10% human serum.

引用

页码：4488 / 4493

页数：6

共 32 条

[11] Mussel-inspired surface chemistry for multifunctional coatings [J].

Lee, Haeshin ;

Dellatore, Shara M. ;

Miller, William M. ;

Messersmith, Phillip B. .

SCIENCE, 2007, 318 (5849) :426-430

[12] Ag/Au bi-metallic film based color surface plasmon resonance biosensor with enhanced sensitivity, color contrast and great linearity [J].

Li, Chung-Tien ;

Lo, Kun-Chi ;

Chang, Hsin-Yun ;

Wu, Hsieh-Ting ;

Ho, Jennifer H. ;

Yen, Ta-Jen .

BIOSENSORS & BIOELECTRONICS, 2012, 36 (01) :192-198

[13] Detection of protein biomarkers using RNA aptamer microarrays and enzymatically amplified surface plasmon resonance imaging [J].

Li, Yuan ;

Lee, Hye Jin ;

Corn, Robert M. .

ANALYTICAL CHEMISTRY, 2007, 79 (03) :1082-1088

[14] Etched Glass Microarrays with Differential Resonance for Enhanced Contrast and Sensitivity of Surface Plasmon Resonance Imaging Analysis [J].

Linman, Matthew J. ;

Abbas, Abdennour ;

Roberts, Christopher C. ;

Cheng, Quan .

ANALYTICAL CHEMISTRY, 2011, 83 (15) :5936-5943

[15] Interface design and multiplexed analysis with surface plasmon resonance (SPR) spectroscopy and SPR imaging [J].

Linman, Matthew J. ;

Abbas, Abdennour ;

Cheng, Quan .

ANALYST, 2010, 135 (11) :2759-2767

[16] Detection of Membrane-Binding Proteins by Surface Plasmon Resonance with an All-Aqueous Amplification Scheme [J].

Liu, Ying ;

Cheng, Quan .

ANALYTICAL CHEMISTRY, 2012, 84 (07) :3179-3186

[17] Highly Sensitive Detection of Protein Toxins by Surface Plasmon Resonance with Biotinylation-Based Inline Atom Transfer Radical Polymerization Amplification [J].

Liu, Ying ;

Dong, Yi ;

Jauw, Jessica ;

Linman, Matthew J. ;

Cheng, Quan .

ANALYTICAL CHEMISTRY, 2010, 82 (09) :3679-3685

[18] Colloidal Au-enhanced surface plasmon resonance immunosensing [J].

Lyon, LA ;

Musick, MD ;

Natan, MJ .

ANALYTICAL CHEMISTRY, 1998, 70 (24) :5177-5183

[19] Surface molecularly imprinted nanowire for protein specific recognition [J].

Ouyang, Ruizhuo ;

Lei, Jianping ;

Ju, Huangxian .

CHEMICAL COMMUNICATIONS, 2008, (44) :5761-5763

[20] Self-Polymerization of Dopamine as a Versatile and Robust Technique to Prepare Polymer Capsules [J].

Postma, Almar ;

Yan, Yan ;

Wang, Yajun ;

Zelikin, Alexander N. ;

Tjipto, Elvira ;

Caruso, Frank .

CHEMISTRY OF MATERIALS, 2009, 21 (14) :3042-3044

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