Encapsulation, Visualization and Expression of Genes with Biomimetically Mineralized Zeolitic Imidazolate Framework-8 (ZIF-8)

被引：138

作者：

Poddar, Arpita ^{[1
,2
]}

Conesa, Jose J. ^{[3
]}

Liang, Kang ^{[4
,5
]}

Dhakal, Sudip ^{[1
]}

Reineck, Philipp ^{[6
]}

Bryant, Gary ^{[7
]}

Pereiro, Eva ^{[3
]}

Ricco, Raffaele ^{[8
]}

Amenitsch, Heinz ^{[9
]}

Doonan, Christian ^{[10
,11
]}

Mulet, Xavier ^{[2
]}

Doherty, Cara M. ^{[2
]}

Falcaro, Paolo ^{[8
]}

Shukla, Ravi ^{[1
]}

机构：

[1] RMIT Univ, Sch Sci, NanoBiotechnol Res Lab NBRL, Ian Potter NanoBiosensing Facil, Melbourne, Vic 3001, Australia

[2] CSIRO Mfg, Clayton, Vic 3168, Australia

[3] ALBA Synchrotron Light Source, MISTRAL Beamline Expt Div, Barcelona 08290, Spain

[4] Univ New South Wales, Sch Chem Engn, Lib Rd, Sydney, NSW 2052, Australia

[5] Univ New South Wales, Grad Sch Biomed Engn, Lib Rd, Sydney, NSW 2052, Australia

[6] RMIT Univ, Sch Sci, ARC Ctr Excellence Nanoscale BioPhoton, Melbourne, Vic 3001, Australia

[7] RMIT Univ, Sch Sci, Ctr Mol & Nanoscale Phys, Melbourne, Vic 3001, Australia

[8] Graz Univ Technol, Inst Phys & Theoret Chem, A-8010 Graz, Austria

[9] Graz Univ Technol, Inst Inorgan Chem, A-8010 Graz, Austria

[10] Univ Adelaide, Dept Chem, Adelaide, SA 5005, Australia

[11] Univ Adelaide, Ctr Adv Nanomat, Adelaide, SA 5005, Australia

来源：

SMALL | 2019年 / 15卷 / 36期

基金：

澳大利亚研究理事会;

关键词：

DNA function; encapsulation; gene therapy; MOFs; transfection; ZIF-8; METAL-ORGANIC FRAMEWORKS; DELIVERY; CHALLENGES; DNA; TOMOGRAPHY; STRATEGIES; THERAPIES; CARRIERS; SYSTEMS;

D O I：

10.1002/smll.201902268

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Recent work in biomolecule-metal-organic framework (MOF) composites has proven to be an effective strategy for the protection of proteins. However, for other biomacromolecules such as nucleic acids, the encapsulation into nano MOFs and the related characterizations are in their infancy. Herein, encapsulation of a complete gene-set in zeolitic imidazolate framework-8 (ZIF-8) MOFs and cellular expression of the gene delivered by the nano MOF composites are reported. Using a green fluorescent protein (GFP) plasmid (plGFP) as a proof-of-concept genetic macromolecule, successful transfection of mammalian cancer cells with plGFP for up to 4 days is shown. Cell transfection assays and soft X-ray cryo-tomography (cryo-SXT) demonstrate the feasibility of DNA@MOF biocomposites as intracellular gene delivery vehicles. Expression occurs over relatively prolonged time points where the cargo nucleic acid is released gradually in order to maintain sustained expression.

引用

页数：7

共 56 条

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