Transferrin-conjugated magnetic silica PLGA nanoparticles loaded with doxorubicin and paclitaxel for brain glioma treatment

被引：317

作者：

Cui, Yanna ^{[1
,2
]}

Xu, Qingxing ^{[2
]}

Chow, Pierce Kah-Hoe ^{[3
,4
,5
]}

Wang, Deping ^{[1
]}

Wang, Chi-Hwa ^{[2
]}

机构：

[1] Tongji Univ, Sch Mat Sci & Engn, Shanghai 201804, Peoples R China

[2] Natl Univ Singapore, Dept Chem & Biomol Engn, Singapore 117576, Singapore

[3] Singapore Gen Hosp, Dept Gen Surg, Singapore 169608, Singapore

[4] Duke NUS Grad Med Sch, Off Clin Sci, Singapore 169857, Singapore

[5] Natl Canc Ctr, Dept Surg Oncol, Singapore 169610, Singapore

来源：

BIOMATERIALS | 2013年 / 34卷 / 33期

基金：

英国医学研究理事会;

关键词：

Magnetic silica PLGA nanoparticles; Transferrin; Doxorubicin; Paclitaxel; Brain glioma; DRUG; DELIVERY; RECEPTOR; CHEMOTHERAPY; INHIBITION; EFFICACY; SYSTEM; AGENTS;

D O I：

10.1016/j.biomaterials.2013.07.075

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The effective treatment of malignant brain glioma is hindered by the poor transport across the blood-brain barrier (BBB) and the low penetration across the blood-tumor barrier (BTB). In this study, transferrin-conjugated magnetic silica PLGA nanoparticles (MNP-MSN-PLGA-Tf NPs) were formulated to overcome these barriers. These NPs were loaded with doxorubicin (DOX) and paclitaxel (PTX), and their anti-proliferative effect was evaluated in vitro and in vivo. The in vitro cytotoxicity of drug-loaded NPs was evaluated in U-87 cells. The delivery and the subsequent cellular uptake of drug-loaded NPs could be enhanced by the presence of magnetic field and the usage of Tf as targeting ligand, respectively. In particular, cells treated with DOX-PTX-NPs-Tf with magnetic field showed the highest cytotoxicity as compared to those treated with DOX-PTX-NPs-Tf, DOX-PTX-NPs, DOX-PTX-NPs-Tf with free Tf. The in vivo therapeutic efficacy of drug-loaded NPs was evaluated in intracranial U-87 MG-luc2 xenograft of BALB/c nude mice. In particular, the DOX-PTX-NPs-Tf treatment exhibited the strongest anti-glioma activity as compared to the PTX-NPs-Tf, DOX-NPs-Tf or DOX-PTX-NPs treatment. Mice did not show acute toxicity after administrating with blank MNP-MSN-PLGA-Tf NPs. Overall, MNP-MSN-PLGA-Tf NPs are promising carriers for the delivery of dual drugs for effective treatment of brain glioma. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：8511 / 8520

页数：10

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