A cell-free approach to accelerate the study of protein-protein interactions in vitro

被引：33
|
作者：
Sierecki, E. [1 ]
Giles, N. [1 ]
Polinkovsky, M. [1 ]
Moustaqil, M. [1 ]
Alexandrov, K. [1 ]
Gambin, Y. [1 ]
机构：
[1] Univ Queensland, Inst Mol Biosci, St Lucia, Qld, Australia
来源：
INTERFACE FOCUS | 2013年 / 3卷 / 05期
基金：
英国医学研究理事会; 澳大利亚国家健康与医学研究理事会;
关键词：
protein-protein interactions; drug discovery; in vitro protein expression; AlphaScreen; single-molecule fluorescence; SMALL-MOLECULE INHIBITORS; P53-MDM2; INTERACTION; P53; DISCOVERY; ASSAY; PATHWAY; COMPLEX; BINDING; SYSTEM; FUTURE;
D O I：
10.1098/rsfs.2013.0018
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Protein-protein interactions are highly desirable targets in drug discovery, yet only a fraction of drugs act as binding inhibitors. Here, we review the different technologies used to find and validate protein-protein interactions. We then discuss how the novel combination of cell-free protein expression, AlphaScreen and single-molecule fluorescence spectroscopy can be used to rapidly map protein interaction networks, determine the architecture of protein complexes, and find new targets for drug discovery.
引用
收藏
页数：10
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