A cell-free approach to accelerate the study of protein-protein interactions in vitro

被引：33

作者：

Sierecki, E. ^{[1
]}

Giles, N. ^{[1
]}

Polinkovsky, M. ^{[1
]}

Moustaqil, M. ^{[1
]}

Alexandrov, K. ^{[1
]}

Gambin, Y. ^{[1
]}

机构：

[1] Univ Queensland, Inst Mol Biosci, St Lucia, Qld, Australia

来源：

INTERFACE FOCUS | 2013年 / 3卷 / 05期

基金：

英国医学研究理事会; 澳大利亚国家健康与医学研究理事会;

关键词：

protein-protein interactions; drug discovery; in vitro protein expression; AlphaScreen; single-molecule fluorescence; SMALL-MOLECULE INHIBITORS; P53-MDM2; INTERACTION; P53; DISCOVERY; ASSAY; PATHWAY; COMPLEX; BINDING; SYSTEM; FUTURE;

D O I：

10.1098/rsfs.2013.0018

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Protein-protein interactions are highly desirable targets in drug discovery, yet only a fraction of drugs act as binding inhibitors. Here, we review the different technologies used to find and validate protein-protein interactions. We then discuss how the novel combination of cell-free protein expression, AlphaScreen and single-molecule fluorescence spectroscopy can be used to rapidly map protein interaction networks, determine the architecture of protein complexes, and find new targets for drug discovery.

引用

页数：10

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