Photoacoustic in vivo 3D imaging of tumor using a highly tumor-targeting probe under high-threshold conditions

被引:4
|
作者
Yamada, Hisatsugu [1 ,3 ]
Matsumoto, Natsuki [1 ]
Komaki, Takanori [1 ]
Konishi, Hiroaki [1 ]
Kimura, Yu [1 ]
Son, Aoi [1 ]
Imai, Hirohiko [2 ]
Matsuda, Tetsuya [2 ]
Aoyama, Yasuhiro [1 ]
Kondo, Teruyuki [1 ]
机构
[1] Kyoto Univ, Grad Sch Engn, Dept Energy & Hydrocarbon Chem, Nishikyo Ku, Kyoto 6158510, Japan
[2] Kyoto Univ, Grad Sch Informat, Dept Syst Sci, Sakyo Ku, Yoshida Honmachi, Kyoto 6068501, Japan
[3] Tokushima Univ, Grad Sch Technol Ind & Social Sci, Tokushima, Japan
关键词
QUANTUM DOTS; FAR-RED; NANOPARTICLES; MICELLES; POLYMERS; CARRIERS; CANCER;
D O I
10.1038/s41598-020-76281-1
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Three-dimensional (3D) representation of a tumor with respect to its size, shape, location, and boundaries is still a challenge in photoacoustic (PA) imaging using artificial contrast agents as probes. We carried out PA imaging of tumors in mice using 800RS-PMPC, which was obtained by coupling of 800RS, a near-infrared cyanine dye, with PMPC, a highly selective tumor-targeting methacrylate polymer having phosphorylcholine side chains, as a probe. The conjugate 800RS-PMPC forms compact nanoparticles (d(DLS)=14.3 nm), retains the biocompatibility of the parent polymer (PMPC) and exhibits unprecedented PA performance. When applied to mice bearing a 6x3x3 mm(3) tumor buried 6 mm beneath the skin, the probe 800RS-PMPC selectively accumulates in the tumor and emits PA signals that are strong enough to be unambiguously distinguished from noise signals of endogenous blood/hemoglobin. The PA image thus obtained under high-threshold conditions allows 3D characterization of the tumor in terms of its size, shape, location, and boundaries.
引用
收藏
页数:9
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