Automated glycan assembly using the Glyconeer 2.1 synthesizer

被引：89

作者：

Hahm, Heung Sik ^{[1
,2
]}

Schlegel, Mark K. ^{[1
,3
,5
]}

Hurevich, Mattan ^{[1
,6
]}

Eller, Steffen ^{[1
,7
]}

Schuhmacher, Frank ^{[1
,2
]}

Hofmann, Johanna ^{[2
,4
]}

Pagel, Kevin ^{[2
,4
]}

Seeberger, Peter H. ^{[1
,2
]}

机构：

[1] Max Planck Inst Colloids & Interfaces, Dept Biomol Syst, D-14476 Potsdam, Germany

[2] Free Univ Berlin, Inst Chem & Biochem, D-14195 Berlin, Germany

[3] GlycoUniverse GmbH & Co KGaA, D-14195 Berlin, Germany

[4] Max Planck Gesell, Fritz Haber Inst, D-14195 Berlin, Germany

[5] Alnylam Pharmaceut, Cambridge, MA 02142 USA

[6] Hebrew Univ Jerusalem, Inst Chem, IL-91904 Jerusalem, Israel

[7] Chemspeed Technol AG, CH-4414 Fullinsdorf, Switzerland

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2017年 / 114卷 / 17期

基金：

欧洲研究理事会;

关键词：

oligosaccharide synthesis; automated synthesis; glycochemistry; glycobiology; Glyconeer; SOLID-PHASE SYNTHESIS; MASS-SPECTROMETRY; PEPTIDE-SYNTHESIS; OLIGOSACCHARIDES;

D O I：

10.1073/pnas.1700141114

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Reliable and rapid access to defined biopolymers by automated DNA and peptide synthesis has fundamentally altered biological research and medical practice. Similarly, the procurement of defined glycans is key to establishing structure-activity relationships and thereby progress in the glycosciences. Here, we describe the rapid assembly of oligosaccharides using the commercially available Glyconeer 2.1 automated glycan synthesizer, monosaccharide building blocks, and a linker-functionalized polystyrene solid support. Purification and quality-control protocols for the oligosaccharide products have been standardized. Synthetic glycans prepared in this way are useful reagents as the basis for glycan arrays, diagnostics, and carbohydrate-based vaccines.

引用

页码：E3385 / E3389

页数：5

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