Initial performance evaluation of a spotted array Mobile Analysis Platform (MAP) for the detection of influenza A/B, RSV, and MERS coronavirus

被引：27

作者：

Hardick, Justin ^{[1
]}

Metzgar, David ^{[2
]}

Risen, Lisa ^{[2
]}

Myers, Christopher ^{[3
]}

Balansay, Melinda ^{[3
]}

Malcom, Trent ^{[4
]}

Rothman, Richard ^{[4
]}

Gaydos, Charlotte ^{[1
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Div Infect Dis, Baltimore, MD 21205 USA

[2] Ibis Biosci, Carlsbad, CA USA

[3] Naval Hlth Res Ctr, San Diego, CA USA

[4] Johns Hopkins Univ, Dept Emergency Med, Baltimore, MD USA

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2018年 / 91卷 / 03期

关键词：

Respiratory viruses; Point-of-care diagnostics; MERS;

D O I：

10.1016/j.diagmicrobio.2018.02.011

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Clinical samples were evaluated with the Mobile Analysis Platform (MAP) to determine platform performance for detecting respiratory viruses in samples previously characterized using clinical reverse transcriptase polymerase chain reaction assays. The percent agreement between MAP and clinical results was 97% for influenza A (73/75), 100% (21/21) for influenza B, 100% (6/6) for respiratory syncytial virus (RSV), and 80% (4/5) for negative specimens. The approximate limit of detection of the MAP was 30 copies/assay for RSV and 1500 copies/assay for Middle East respiratory syndrome coronavirus. (C) 2018 Elsevier Inc. All rights reserved.

引用

页码：245 / 247

页数：3

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