N-Linked Glycans of Chloroviruses Sharing a Core Architecture without Precedent

被引：33

作者：

De Castro, Cristina ^{[1
]}

Speciale, Immacolata ^{[2
]}

Duncan, Garry ^{[3
]}

Dunigan, David D. ^{[4
,5
]}

Agarkova, Irina ^{[4
,5
]}

Lanzetta, Rosa ^{[2
]}

Sturiale, Luisa ^{[6
]}

Palmigiano, Angelo ^{[6
]}

Garozzo, Domenico ^{[6
]}

Molinaro, Antonio ^{[2
]}

Tonetti, Michela ^{[7
,8
]}

Van Etten, James L. ^{[4
,5
]}

机构：

[1] Univ Naples Federico II, Dept Agr Sci, I-80055 Portici, NA, Italy

[2] Univ Naples Federico II, Dept Chem Sci, I-80126 Naples, Italy

[3] Nebraska Wesleyan Univ, Dept Biol, Lincoln, NE USA

[4] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA

[5] Univ Nebraska, Nebraska Ctr Virol, Lincoln, NE 68583 USA

[6] CNR, Inst Polymers Composites & Biomat, I-95126 Catania, Italy

[7] Univ Genoa, Dept Expt Med, I-16132 Genoa, Italy

[8] Univ Genoa, Ctr Excellence Biomed Res, I-16132 Genoa, Italy

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2016年 / 55卷 / 02期

基金：

美国国家科学基金会;

关键词：

glycoproteins; glycosylations; natural products; structural biology; viruses; MAJOR CAPSID PROTEIN; GLYCOSYLATION;

D O I：

10.1002/anie.201509150

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

N-glycosylation is a fundamental modification of proteins and exists in the three domains of life and in some viruses, including the chloroviruses, for which a new type of core N-glycan is herein described. This N-glycan core structure, common to all chloroviruses, is a pentasaccharide with a beta-glucose linked to an asparagine residue which is not located in the typical sequon N-X-T/S. The glucose is linked to a terminal xylose unit and a hyperbranched fucose, which is in turn substituted with a terminal galactose and a second xylose residue. The third position of the fucose unit is always linked to a rhamnose, which is a semiconserved element because its absolute configuration is virus-dependent. Additional decorations occur on this core N-glycan and represent a molecular signature for each chlorovirus.

引用

页码：654 / 658

页数：5

共 12 条

[1] Structure of N-linked oligosaccharides attached to chlorovirus PBCV-1 major capsid protein reveals unusual class of complex N-glycans [J].