Characterization of a Hemolysin Gene ytjA from Bacillus subtilis

被引：16

作者：

Liu, Jie ^{[1
]}

Fang, Chunhong ^{[1
]}

Jiang, Yanlong ^{[2
]}

Yan, Ruoyu ^{[1
]}

机构：

[1] NW A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi Prov, Peoples R China

[2] NE Agr Univ, Coll Life Sci, Harbin 150030, Heilongjiang Pr, Peoples R China

来源：

CURRENT MICROBIOLOGY | 2009年 / 58卷 / 06期

关键词：

STAPHYLOCOCCAL ALPHA-TOXIN; ESCHERICHIA-COLI; STREPTOLYSIN-O; VIRULENCE; THURINGIENSIS; PURIFICATION; SURFACTIN; SEQUENCE; CEREUS; TRACT;

D O I：

10.1007/s00284-009-9383-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bacillus subtilis is popularly used as a probiotic in many fields. Although recent research has found that some secondary metabolites of B. subtilis could cause hemolysis, the hemolytic mechanism in B. subtilis is still unclear. In this paper, the hemolysis-associated gene ytjA was cloned and expressed in Escherichia coli, and the hemolytic activity of the expressed soluble protein was indicated by the presence of clear hemolytic zones on sheep blood agar plates. In addition, reverse transcriptase-polymerase chain reaction analysis confirmed that the ytjA gene was transcribed in B. subtilis. These results suggest that ytjA is one of the hemolysin genes responsible for hemolysis in B. subtilis.

引用

页码：642 / 647

页数：6

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