Amifostine Pretreatment Attenuates Myocardial Ischemia/Reperfusion Injury by Inhibiting Apoptosis and Oxidative Stress

被引：34
作者：
Wu, Shao-ze
Tao, Lu-yuan
Wang, Jiao-ni
Xu, Zhi-qiang
Wang, Jie
Xue, Yang-jing
Huang, Kai-yu
Lin, Jia-feng
Li, Lei
Ji, Kang-ting [1 ]
机构：
[1] Wenzhou Med Univ, Affiliated Hosp 2, Dept Cardiol, Wenzhou 325000, Peoples R China
来源：
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY | 2017年 / 2017卷
基金：
中国国家自然科学基金;
关键词：
ISCHEMIA-REPERFUSION; MITOCHONDRIAL DYSFUNCTION; MECHANISMS; RADIATION; PROTECTS; CELLS;
D O I：
10.1155/2017/4130824
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The present study was aimed at investigating the effect of amifostine on myocardial ischemia/reperfusion (I/R) injury of mice and H9c2 cells cultured with TBHP (tert-butyl hydroperoxide). The results showed that pretreatment with amifostine significantly attenuated cell apoptosis and death, accompanied by decreased reactive oxygen species (ROS) production and lower mitochondrial potential (Delta psi m). In vivo, amifostine pretreatment alleviated I/R injury and decreasedmyocardial apoptosis and infarct area, which was paralleled by increased superoxide dismutase (SOD) and reduced malondialdehyde (MDA) in myocardial tissues, increased Bcl2 expression, decreased Bax expression, lower cleaved caspase-3 level, fewer TUNEL positive cells, and fewer DHE-positive cells in heart. Our results indicate that amifostine pretreatment has a protective effect againstmyocardial I/R injury via scavenging ROS.
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页数：12
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