Crystal structure of the zymogen form of the group A Streptococcus virulence factor SpeB:: An integrin-binding cysteine protease

被引:91
作者
Kagawa, TF
Cooney, JC
Baker, HM
McSweeney, S
Liu, MY
Gubba, S
Musser, JM
Baker, EN
机构
[1] Univ Auckland, Sch Biol Sci, Auckland, New Zealand
[2] Univ Auckland, Dept Chem, Auckland, New Zealand
[3] Massey Univ, Inst Mol Biosci, Palmerston North, New Zealand
[4] European Mol Biol Lab, Grenoble Outstn, F-38042 Grenoble, France
[5] Baylor Coll Med, Dept Pathol, Inst Human Bacterial Pathogenesis, Houston, TX 77030 USA
[6] NIAID, Rocky Mt Labs, Lab Human Bacterial Pathogenesis, NIH, Hamilton, MT 59840 USA
关键词
D O I
10.1073/pnas.040549997
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Pathogenic bacteria secrete protein toxins that weaken or disable their host, and thereby act as virulence factors. We have determined the crystal structure of streptococcal pyrogenic exotoxin B (SpeB), a cysteine protease that is a major virulence factor of the human pathogen Streptococcus pyogenes and participates in invasive disease episodes, including necrotizing fasciitis. The structure, determined for the 40-kDa precursor form of SpeB at 1.6-Angstrom, resolution, reveals that the protein is a distant homologue of the papain superfamily that includes the mammalian cathepsins B, K, L, and S. Despite negligible sequence identity, the protease portion has the canonical papain fold, albeit with major loop insertions and deletions. The catalytic site differs from most other cysteine proteases in that it lacks the Asn residue of the Cys-His-Asn triad. The prosegment has a unique fold and inactivation mechanism that involves displacement of the catalytically essential His residue by a loop inserted into the active site. The structure also reveals the surface location of an integrin-binding Arg-Gly-Asp (RGD) motif that is a feature unique to SpeB among cysteine proteases and is linked to the pathogenesis of the most invasive strains of S. pyogenes.
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页码:2235 / 2240
页数:6
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