One-step detection of pathogens and cancer biomarkers by the naked eye based on aggregation of immunomagnetic beads

被引：41
作者：
Chen, Yiping [1 ,2 ]
Xianyu, Yunlei [1 ,2 ]
Sun, Jiashu [1 ,2 ]
Niu, Yajing [1 ,2 ]
Wang, Yu [3 ]
Jiang, Xingyu [1 ,2 ]
机构：
[1] Natl Ctr NanoSci & Technol, Beijing Engn Res Ctr BioNanotechnol, Beijing 100190, Peoples R China
[2] Natl Ctr NanoSci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China
[3] Capital Med Univ, Beijing Friendship Hosp, Beijing Inst Trop Med, Beijing 100050, Peoples R China
来源：
NANOSCALE | 2016年 / 8卷 / 02期
基金：
中国国家自然科学基金;
关键词：
ULTRASENSITIVE DETECTION; CARE DIAGNOSTICS; SMALL MOLECULES; POINT; DNA; ASSAY;
D O I：
10.1039/c5nr07044a
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
This report shows that immunomagnetic beads (IMBs) can act as the optical readout for assays, in addition to serving as the carrier for purification/separation. Under the influence of an external magnet, IMBs are attracted to coat one side of a test tube. IMBs specifically bound to targets can form a narrow brown stripe, whereas free IMBs will form a diffuse, yellow coating on the side of the test tube. Target analytes can aggregate initially dispersed IMBs in a sample concentration-dependent manner, yielding a color change from yellow to brown that can be seen with the naked eye. This assay combines the convenience of a lateral flow assay, allowing a one-step assay to finish within 15 min, with the sensitivity of an enzyme-linked immonosorbent assay.
引用
收藏
页码：1100 / 1107
页数：8
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