One-step detection of pathogens and cancer biomarkers by the naked eye based on aggregation of immunomagnetic beads

被引：41

作者：

Chen, Yiping ^{[1
,2
]}

Xianyu, Yunlei ^{[1
,2
]}

Sun, Jiashu ^{[1
,2
]}

Niu, Yajing ^{[1
,2
]}

Wang, Yu ^{[3
]}

Jiang, Xingyu ^{[1
,2
]}

机构：

[1] Natl Ctr NanoSci & Technol, Beijing Engn Res Ctr BioNanotechnol, Beijing 100190, Peoples R China

[2] Natl Ctr NanoSci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China

[3] Capital Med Univ, Beijing Friendship Hosp, Beijing Inst Trop Med, Beijing 100050, Peoples R China

来源：

NANOSCALE | 2016年 / 8卷 / 02期

基金：

中国国家自然科学基金;

关键词：

ULTRASENSITIVE DETECTION; CARE DIAGNOSTICS; SMALL MOLECULES; POINT; DNA; ASSAY;

D O I：

10.1039/c5nr07044a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This report shows that immunomagnetic beads (IMBs) can act as the optical readout for assays, in addition to serving as the carrier for purification/separation. Under the influence of an external magnet, IMBs are attracted to coat one side of a test tube. IMBs specifically bound to targets can form a narrow brown stripe, whereas free IMBs will form a diffuse, yellow coating on the side of the test tube. Target analytes can aggregate initially dispersed IMBs in a sample concentration-dependent manner, yielding a color change from yellow to brown that can be seen with the naked eye. This assay combines the convenience of a lateral flow assay, allowing a one-step assay to finish within 15 min, with the sensitivity of an enzyme-linked immonosorbent assay.

引用

页码：1100 / 1107

页数：8

共 23 条

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