One-step detection of pathogens and cancer biomarkers by the naked eye based on aggregation of immunomagnetic beads

被引：41

作者：

Chen, Yiping ^{[1
,2
]}

Xianyu, Yunlei ^{[1
,2
]}

Sun, Jiashu ^{[1
,2
]}

Niu, Yajing ^{[1
,2
]}

Wang, Yu ^{[3
]}

Jiang, Xingyu ^{[1
,2
]}

机构：

[1] Natl Ctr NanoSci & Technol, Beijing Engn Res Ctr BioNanotechnol, Beijing 100190, Peoples R China

[2] Natl Ctr NanoSci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China

[3] Capital Med Univ, Beijing Friendship Hosp, Beijing Inst Trop Med, Beijing 100050, Peoples R China

来源：

NANOSCALE | 2016年 / 8卷 / 02期

基金：

中国国家自然科学基金;

关键词：

ULTRASENSITIVE DETECTION; CARE DIAGNOSTICS; SMALL MOLECULES; POINT; DNA; ASSAY;

D O I：

10.1039/c5nr07044a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This report shows that immunomagnetic beads (IMBs) can act as the optical readout for assays, in addition to serving as the carrier for purification/separation. Under the influence of an external magnet, IMBs are attracted to coat one side of a test tube. IMBs specifically bound to targets can form a narrow brown stripe, whereas free IMBs will form a diffuse, yellow coating on the side of the test tube. Target analytes can aggregate initially dispersed IMBs in a sample concentration-dependent manner, yielding a color change from yellow to brown that can be seen with the naked eye. This assay combines the convenience of a lateral flow assay, allowing a one-step assay to finish within 15 min, with the sensitivity of an enzyme-linked immonosorbent assay.

引用

页码：1100 / 1107

页数：8

共 23 条

[1] Single-Step Homogeneous Immunoassays Utilizing Epitope-Tagged Gold Nanoparticles: On the Mechanism, Feasibility, and Limitations
Andresen, Heiko
Mager, Morgan
Griessner, Matthias
Charchar, Patrick
Todorova, Nevena
Bell, Nia
Theocharidis, Georgios
Bertazzo, Sergio
Yarovsky, Irene
Stevens, Molly M.
[J]. CHEMISTRY OF MATERIALS, 2014, 26 (16) : 4696 - 4704
[2] Computational Lateral Flow Biosensor for Proteins and Small Molecules: A New Class of Strip Logic Gates
Chen, Junhua
Fang, Zhiyuan
Lie, Puchang
Zeng, Lingwen
[J]. ANALYTICAL CHEMISTRY, 2012, 84 (15) : 6321 - 6325
[3] Chung HJ, 2013, NAT NANOTECHNOL, V8, P369, DOI [10.1038/NNANO.2013.70, 10.1038/nnano.2013.70]
[4] de la Rica R, 2012, NAT NANOTECHNOL, V7, P821, DOI [10.1038/nnano.2012.186, 10.1038/NNANO.2012.186]
[5] Point of Care Diagnostics: Status and Future
Gubala, Vladimir
Harris, Leanne F.
Ricco, Antonio J.
Tan, Ming X.
Williams, David E.
[J]. ANALYTICAL CHEMISTRY, 2012, 84 (02) : 487 - 515
[6] Kinase-Actuated Immunoaggregation of Peptide-Conjugated Gold Nanoparticles
Gupta, Shalini
Andresen, Heiko
Ghadiali, James E.
Stevens, Molly M.
[J]. SMALL, 2010, 6 (14) : 1509 - 1513
[7] Virus-Tethered Magnetic Gold Microspheres with Biomimetic Architectures for Enhanced Immunoassays
Jeon, Chang Su
Hwang, Inseong
Chung, Taek Dong
[J]. ADVANCED FUNCTIONAL MATERIALS, 2013, 23 (12) : 1484 - 1489
[8] New Detection Modality for Label-Free Quantification of DNA in Biological Samples via Superparamagnetic Bead Aggregation
Leslie, Daniel C.
Li, Jingyi
Strachan, Briony C.
Begley, Matthew R.
Finkler, David
Bazydlo, Lindsay A. L.
Barker, N. Scott
Haverstick, Doris M.
Utz, Marcel
Landers, James P.
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2012, 134 (12) : 5689 - 5696
[9] A Highly Sensitive Gold-Nanoparticle-Based Assay for Acetylcholinesterase in Cerebrospinal Fluid of Transgenic Mice with Alzheimer's Disease
Liu, Dingbin
Chen, Wenwen
Tian, Yue
He, Sha
Zheng, Wenfu
Sun, Jiashu
Wang, Zhuo
Jiang, Xingyu
[J]. ADVANCED HEALTHCARE MATERIALS, 2012, 1 (01) : 90 - 95
[10] Ultrasensitive Detection of Cancer Biomarkers in the Clinic by Use of a Nanostructured Microfluidic Array
Malhotra, Ruchika
Patel, Vyomesh
Chikkaveeraiah, Bhaskara V.
Munge, Bernard S.
Cheong, Sok Ching
Zain, Rosnah B.
Abraham, Mannil T.
Dey, Dipak K.
Gutkind, J. Silvio
Rusling, James F.
[J]. ANALYTICAL CHEMISTRY, 2012, 84 (14) : 6249 - 6255

← 1 2 3 →