Sex-related effects of imidacloprid modulated by piperonyl butoxide and menadione in rats. Part II: genotoxic and cytotoxic potential

被引：21

作者：

Arslan, Mehmet ^{[1
]}

Sevgiler, Yusuf ^{[2
]}

Buyukleyla, Mehmet ^{[3
]}

Yardimci, Mustafa ^{[4
]}

Yilmaz, Mehmet ^{[3
]}

Rencuzogullari, Eyyup ^{[2
]}

机构：

[1] Ardahan Univ, Sch Hlth Sci, Dept Nursing, TR-02040 Ardahan, Turkey

[2] Adiyaman Univ, Fac Sci & Letters, Dept Biol, TR-02040 Adiyaman, Turkey

[3] Cukurova Univ, Inst Nat & Appl Sci, Dept Biol, Adana, Turkey

[4] Adiyaman Univ, Inst Nat & Appl Sci, Dept Biol, TR-02040 Adiyaman, Turkey

来源：

DRUG AND CHEMICAL TOXICOLOGY | 2016年 / 39卷 / 01期

关键词：

Cytotoxicity; genotoxicity; imidacloprid; menadione; piperonyl butoxide; rats; sex; structural chromosomal aberrations; NICOTINIC ACETYLCHOLINE-RECEPTOR; HEPATIC ALDEHYDE OXIDASE; DNA-DAMAGE; IN-VITRO; OXIDATIVE STRESS; BONE-MARROW; COMET ASSAY; TOXICITY; METABOLISM; PESTICIDES;

D O I：

10.3109/01480545.2015.1029049

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Despite its intended use, imidacloprid causes genotoxic and cytotoxic effects in mammals, especially in the presence of metabolic activation systems. The aim of this study was to determine to which extent these effects are sex related and how its metabolism modulators piperonyl butoxide and menadione affect its toxicity. Male and female Sprague-Dawley rats were injected with the intraperitoneal LD50 dose of imidacloprid alone (170mg/kg) or pretreated with piperonyl butoxide (100mg/kg) and menadione (25mg/kg) for 12 and 24h. Structural chromosome aberrations, abnormal cells and mitotic index were determined microscopically in bone marrow cells. Male rats showed susceptibility to the genotoxic effects of imidacloprid. Piperonyl butoxide was effective in countering this effect only at 24h, whereas menadione exacerbated imidacloprid-induced genotoxicity. Piperonyl butoxide and menadione pretreatments increased the percentage of structural chromosome aberrations and abnormal cells in females. Imidacloprid decreased the mitotic index, whereas pretreatment with piperonyl butoxide and menadione showed improvement in both sexes. We believe that CYP450-mediated metabolism of imidacloprid is under the hormonal control and therefore that its genotoxicity is sex related. Piperonyl butoxide pretreatment also showed sex-related modulation. The hormonal effects on imidacloprid biotransformation require further investigation.

引用

页码：81 / 86

页数：6

共 47 条

[31] Imidacloprid insecticide metabolism: human cytochrome P450 isozymes differ in selectivity for imidazolidine oxidation versus nitroimine reduction [J].

Schulz-Jander, DA ;

Casida, JE .

TOXICOLOGY LETTERS, 2002, 132 (01) :65-70

[32] Pro-oxidant Induced DNA Damage in Human Lymphoblastoid Cells: Homeostatic Mechanisms of Genotoxic Tolerance [J].

Seager, Anna L. ;

Shah, Ume-Kulsoom ;

Mikhail, Jane M. ;

Nelson, Bryant C. ;

Marquis, Bryce J. ;

Doak, Shareen H. ;

Johnson, George E. ;

Griffiths, Sioned M. ;

Carmichael, Paul L. ;

Scott, Sharon J. ;

Scott, Andrew D. ;

Jenkins, Gareth J. S. .

TOXICOLOGICAL SCIENCES, 2012, 128 (02) :387-397

[33] Determination of genotoxicity of the metabolites of the pesticides Guthion, Sencor, Lorox, Reglone, Daconil and Admire by P-32-postlabeling [J].

Shah, RG ;

Lagueux, J ;

Kapur, S ;

Levallois, P ;

Ayotte, P ;

Tremblay, M ;

Zee, J ;

Poirier, GG .

MOLECULAR AND CELLULAR BIOCHEMISTRY, 1997, 169 (1-2) :177-184

[34] Dose- and time-dependent effects of menadione on enzymes of xenobiotic metabolism in rat liver [J].

Sidorova, YA ;

Grishanova, AY .

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE, 2004, 137 (03) :231-234

[35] Induction of glutathione S-transferase activity in Triatoma infestans [J].

Sivori, JL ;

Casabe, N ;

Zerba, EN ;

Wood, EJ .

MEMORIAS DO INSTITUTO OSWALDO CRUZ, 1997, 92 (06) :797-802

[36] EFFECTS OF PIPERONYL BUTOXIDE ON HALOTHANE HEPATOTOXICITY AND METABOLISM IN THE HYPERTHYROID RAT [J].

SMITH, AC ;

ROBERTS, SM ;

BERMAN, LM ;

HARRISON, RD ;

JAMES, RC .

TOXICOLOGY, 1988, 50 (01) :95-105

[37] Imidacloprid, thiacloprid, and their imine derivatives up-regulate the α4β2 nicotinic acetylcholine receptor in M10 cells [J].

Tomizawa, M ;

Casida, JE .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 2000, 169 (01) :114-120

[38] Neonicotinoid insecticide toxicology: Mechanisms of selective action [J].

Tomizawa, M ;

Casida, JE .

ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 2005, 45 :247-+

[39] Selective toxicity of neonicotinoids attributable to specificity of insect and mammalian nicotinic receptors [J].

Tomizawa, M ;

Casida, JE .

ANNUAL REVIEW OF ENTOMOLOGY, 2003, 48 :339-364

[40] In vivo chromosomal aberrations in bone marrow cells of rats treated with Marshal [J].

Topaktas, M ;

Rencuzogullari, E ;

Ila, HB .

MUTATION RESEARCH-GENETIC TOXICOLOGY, 1996, 371 (3-4) :259-264

← 1 2 3 4 5 →