Trimmomatic: a flexible trimmer for Illumina sequence data

被引:44344
作者
Bolger, Anthony M. [1 ,2 ]
Lohse, Marc [1 ]
Usadel, Bjoern [2 ,3 ]
机构
[1] Max Planck Inst Mol Plant Physiol, Dept Metab Networks, D-14476 Golm, Germany
[2] Rhein Westfal TH Aachen, Inst Biol 3, D-52074 Aachen, Germany
[3] Forschungszentrum Julich, Inst Bio & Geosci Plant Sci, D-52425 Julich, Germany
关键词
READ ALIGNMENT; ALGORITHMS;
D O I
10.1093/bioinformatics/btu170
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Motivation: Although many next-generation sequencing (NGS) read preprocessing tools already existed, we could not find any tool or combination of tools that met our requirements in terms of flexibility, correct handling of paired-end data and high performance. We have developed Trimmomatic as a more flexible and efficient preprocessing tool, which could correctly handle paired-end data. Results: The value of NGS read preprocessing is demonstrated for both reference-based and reference-free tasks. Trimmomatic is shown to produce output that is at least competitive with, and in many cases superior to, that produced by other tools, in all scenarios tested.
引用
收藏
页码:2114 / 2120
页数:7
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