Cryopreservation of trout primordial germ cells

被引：20

作者：

Kobayashi, T

Takeuchi, Y

Yoshizaki, G

Takeuchi, T

机构：

[1] Tokyo Univ Marine Sci & Technol, Dept Marine Biosci, Minato Ku, Tokyo 1088477, Japan

[2] Japan Sci & Technol Corp, PRESTO, Kawaguchi, Japan

来源：

FISH PHYSIOLOGY AND BIOCHEMISTRY | 2003年 / 28卷 / 1-4期

关键词：

D O I：

10.1023/B:FISH.0000030634.37486.fa

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for cryopreserving trout primordial germ cells (PGCs) was established. Trout PGCs were successfully cryopreserved with high survival by freezing with medium containing 1.8 M ethylene glycol. Transplantation experiment revealed that frozen/thawed PGCs colonized and proliferated in the recipients' gonads.

引用

页码：479 / 480

页数：2