Simultaneous quantification of protein order and disorder

被引:101
作者
Sormanni, Pietro [1 ]
Piovesan, Damiano [2 ,3 ]
Heller, Gabriella T. [1 ]
Bonomi, Massimiliano [1 ]
Kukic, Predrag [1 ]
Camilloni, Carlo [4 ,5 ]
Fuxreiter, Monika [6 ]
Dosztanyi, Zsuzsanna [7 ]
Pappu, Rohit V. [8 ,9 ]
Babu, M. Madan [10 ]
Longhi, Sonia [11 ]
Tompa, Peter [12 ,13 ]
Dunker, A. Keith [14 ,15 ]
Uversky, Vladimir N. [16 ,17 ]
Tosatto, Silvio C. E. [2 ,3 ]
Vendruscolo, Michele [1 ]
机构
[1] Univ Cambridge, Dept Chem, Cambridge, England
[2] Univ Padua, Dept Biomed Sci, Padua, Italy
[3] Univ Padua, CRIBI Biotechnol Ctr, Padua, Italy
[4] Tech Univ Munich, Dept Chem, Garching, Germany
[5] Tech Univ Munich, Inst Adv Study, Garching, Germany
[6] Univ Debrecen, Dept Biochem & Mol Biol, MTA Momentum Lab Prot Dynam, Debrecen, Hungary
[7] Eotvos Lorand Univ, MTA ELTE Lendulet Bioinformat Res Grp, Dept Biochem, Budapest, Hungary
[8] Washington Univ, Dept Biomed Engn, St Louis, MO USA
[9] Washington Univ, Ctr Biol Syst Engn, St Louis, MO USA
[10] MRC Lab Mol Biol, Cambridge, England
[11] Aix Marseille Univ, AFMB, CNRS, Marseille, France
[12] Vrije Univ Brussel, VIB Ctr Struct Biol, Brussels, Belgium
[13] Inst Enzymol, Budapest, Hungary
[14] Indiana Univ, Sch Med, Dept Biochem & Mol Biol, Ctr Computat Biol & Bioinformat, Indianapolis, IN USA
[15] Indiana Univ, Sch Informat, Dept Biochem & Mol Biol, Ctr Computat Biol & Bioinformat, Indianapolis, IN USA
[16] Univ S Florida, Morsani Coll Med, Dept Mol Biol, Tampa, FL USA
[17] Univ S Florida, Morsani Coll Med, USF Hlth Byrd Alzheimers Res Inst, Tampa, FL USA
关键词
STRUCTURAL BIOLOGY; ATOMIC-RESOLUTION; CHEMICAL-SHIFTS; NMR; STATES; POPULATIONS; DYNAMICS; REGIONS;
D O I
10.1038/nchembio.2331
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Nuclear magnetic resonance spectroscopy is transforming our views of proteins by revealing how their structures and dynamics are closely intertwined to underlie their functions and interactions. Compelling representations of proteins as statistical ensembles are uncovering the presence and biological relevance of conformationally heterogeneous states, thus gradually making it possible to go beyond the dichotomy between order and disorder through more quantitative descriptions that span the continuum between them.
引用
收藏
页码:339 / 342
页数:4
相关论文
共 25 条
[1]   NMR spectroscopy brings invisible protein states into focus [J].
Baldwin, Andrew J. ;
Kay, Lewis E. .
NATURE CHEMICAL BIOLOGY, 2009, 5 (11) :808-814
[2]   Finding Our Way in the Dark Proteome [J].
Bhowmick, Asmit ;
Brookes, David H. ;
Yost, Shane R. ;
Dyson, H. Jane ;
Forman-Kay, Julie D. ;
Gunter, Daniel ;
Head-Gordon, Martin ;
Hura, Gregory L. ;
Pande, Vijay S. ;
Wemmer, David E. ;
Wright, Peter E. ;
Head-Gordon, Teresa .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2016, 138 (31) :9730-9742
[3]   Metainference: A Bayesian inference method for heterogeneous systems [J].
Bonomi, Massimiliano ;
Camilloni, Carlo ;
Cavalli, Andrea ;
Vendruscolo, Michele .
SCIENCE ADVANCES, 2016, 2 (01)
[4]   A structural ensemble of a ribosome-nascent chain complex during cotranslational protein folding [J].
Cabrita, Lisa D. ;
Cassaignau, Anais M. E. ;
Launay, Helene M. M. ;
Waudby, Christopher A. ;
Wlodarski, Tomasz ;
Camilloni, Carlo ;
Karyadi, Maria-Evangelia ;
Robertson, Amy L. ;
Wang, Xiaolin ;
Wentink, Anne S. ;
Goodsell, Luke S. ;
Woolhead, Cheryl A. ;
Vendruscolo, Michele ;
Dobson, Christopher M. ;
Christodoulou, John .
NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2016, 23 (04) :278-285
[5]   THE REVOLUTION WILL NOT BE CRYSTALLIZED [J].
Callaway, Ewen .
NATURE, 2015, 525 (7568) :172-174
[6]   Cyclophilin A catalyzes proline isomerization by an electrostatic handle mechanism [J].
Camilloni, Carlo ;
Sahakyan, Aleksandr B. ;
Holliday, Michael J. ;
Isern, Nancy G. ;
Zhang, Fengli ;
Eisenmesser, Elan Z. ;
Vendruscolo, Michele .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2014, 111 (28) :10203-10208
[7]   Determination of Secondary Structure Populations in Disordered States of Proteins Using Nuclear Magnetic Resonance Chemical Shifts [J].
Camilloni, Carlo ;
De Simone, Alfonso ;
Vranken, Wim F. ;
Vendruscolo, Michele .
BIOCHEMISTRY, 2012, 51 (11) :2224-2231
[8]   Protein structure determination from NMR chemical shifts [J].
Cavalli, Andrea ;
Salvatella, Xavier ;
Dobson, Christopher M. ;
Vendruscolo, Michele .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (23) :9615-9620
[9]   Structure of a low-population intermediate state in the release of an enzyme product [J].
De Simone, Alfonso ;
Aprile, Francesco A. ;
Dhulesia, Anne ;
Dobson, Christopher M. ;
Vendruscolo, Michele .
ELIFE, 2015, 4
[10]   Intrinsically unstructured proteins and their functions [J].
Dyson, HJ ;
Wright, PE .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2005, 6 (03) :197-208