Phage-Type RNA Polymerase RPOTmp Performs Gene-Specific Transcription in Mitochondria of Arabidopsis thaliana

被引：107

作者：

Kuehn, Kristina ^{[1
]}

Richter, Uwe ^{[2
]}

Meyer, Etienne H. ^{[1
]}

Delannoy, Etienne ^{[1
]}

de Longevialle, Andeol Falcon ^{[1
]}

O'Toole, Nicholas ^{[3
]}

Boerner, Thomas ^{[2
]}

Millar, A. Harvey ^{[1
]}

Small, Ian D. ^{[1
,3
]}

Whelan, James ^{[1
]}

机构：

[1] Univ Western Australia, Australian Res Council, Ctr Excellence Plant Energy Biol, Crawley, WA 6009, Australia

[2] Humboldt Univ, Inst Biol Genet, D-10115 Berlin, Germany

[3] Univ Western Australia, Ctr Computat Syst Biol, Crawley, WA 6009, Australia

来源：

PLANT CELL | 2009年 / 21卷 / 09期

基金：

澳大利亚研究理事会;

关键词：

COMPLETE NUCLEOTIDE-SEQUENCE; HIGHER-PLANTS; COX2; GENE; PROMOTER; INITIATION; NUCLEAR; GENOME; EXPRESSION; IDENTIFICATION; CONTAINS;

D O I：

10.1105/tpc.109.068536

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transcription of mitochondrial genes in animals, fungi, and plants relies on the activity of T3/T7 phage-type RNA polymerases. Two such enzymes, RPOTm and RPOTmp, are present in the mitochondria of eudicotyledonous plants; RPOTmp is additionally found in plastids. We have characterized the transcriptional role of the dual-targeted RNA polymerase in mitochondria of Arabidopsis thaliana. Examination of mitochondrial transcripts in rpoTmp mutants revealed major differences in transcript abundances between wild-type and rpoTmp plants. Decreased levels of specific transcripts were correlated with reduced abundances of the respiratory chain complexes I and IV. Altered transcript levels in rpoTmp were found to result from gene-specific transcriptional changes, establishing that RPOTmp functions in distinct transcriptional processes within mitochondria. Decreased transcription of specific genes in rpoTmp was not associated with changes in promoter utilization; therefore, RPOTmp function is not promoter specific but gene specific. This implies that additional gene-specific elements direct the transcription of a subset of mitochondrial genes by RPOTmp.

引用

页码：2762 / 2779

页数：18

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