Synthesis of Nanohydroxyapatite from Cuttlefish Bone (Sepia sp.) Using Milling Method

被引:8
作者
Aminatun [1 ]
Supardi, Adri [1 ]
Nisa, Zulifah Izzatin [1 ]
Hikmawati, Dyah [1 ]
Siswanto [1 ]
机构
[1] Univ Airlangga, Dept Phys, Fac Sci & Technol, Surabaya 60115, Indonesia
关键词
D O I
10.1155/2019/1831208
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
The synthesis of nanohydroxyapatite from cuttlefish bone (Sepia sp.) has been done by using High Energy Milling (HEM) and its characterization in vitro as bone repair. This study aimed to determine the effect of the milling process on microscopic properties and mechanical properties of nano-HA through XRD, TEM, and compressive strength tests. The hydroxyapatite (HA) used in this study consisted of 1M CaCO3 from aragonite which was extracted from cuttlefish lamella bone (Sepia sp.) and 0.6 M NH4H2PO4, which was hydrothermally processed at 200 degrees C for 12 h and then sintered at 900 degrees C for 1h. Parameter milling includes the variation of milling time, i.e., 3 h, 6 h, and 9 h at rotational speed of 350 rpm. An increase in milling time causes a decrease in HA particle size. This is shown from the results of TEM at the milling time of 9 h with the smallest size up to 65 nm. The result of cell viability test showed that all samples are not toxic with cell viability value of >80%. The milling time of 9 h was an optimum condition with a compressive strength of 4.35952 MPa that can be applied to cancellous bone.
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页数:6
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