Expression profiling of zebrafish sox9 mutants reveals that Sox9 is required for retinal differentiation

被引:45
作者
Yokoi, Hayato [1 ]
Yan, Yi-Lin [1 ]
Miller, Michael R. [1 ,2 ]
BreMiller, Ruth A. [1 ]
Catchen, Julian M. [1 ,3 ]
Johnson, Eric A. [2 ]
Postlethwait, John H. [1 ]
机构
[1] Univ Oregon, Inst Neurosci, Eugene, OR 97403 USA
[2] Univ Oregon, Inst Mol Biol, Eugene, OR 97403 USA
[3] Univ Oregon, Dept Comp & Informat Sci, Eugene, OR 97403 USA
基金
美国国家卫生研究院;
关键词
Col11a2; Calb2; Transcription factor; Evolution of gene function; Microarray; Muller glia; Photoreceptor; II COLLAGEN GENE; CHONDROCYTE-SPECIFIC ENHANCER; BIPOLAR CELL-DIFFERENTIATION; VERTEBRATE GENOME EVOLUTION; HELIX TRANSCRIPTION FACTOR; NEURAL CREST DEVELOPMENT; AUTOSOMAL SEX REVERSAL; X-LINKED RETINOSCHISIS; SRY-RELATED GENE; HOMEOBOX GENE;
D O I
10.1016/j.ydbio.2009.01.002
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The transcription factor gene Sox9 plays various roles in development, including differentiation of the skeleton, gonads, glia, and heart. Other functions of Sox9 remain enigmatic. Because Sox9 protein regulates expression of target genes, the identification of Sox9 targets should facilitate an understanding of the mechanisms of Sox9 action. To help identify Sox9 targets, we used microarray expression profiling to compare wild-type embryos to mutant embryos lacking activity for both sox9a and sox9b, the zebrafish co-orthologs of Sox9. Candidate genes were further evaluated by whole-mount in situ hybridization in wild-type and sox9 single and double mutant embryos. Results identified genes expressed in cartilage (col2a1a and col11a2), retina (calb2a, calb2b, crx, neurod, rs1, sox4a and vsx1) and pectoral fin bud (klf2b and EST Al722369) as candidate targets for Sox9. Cartilage is a well-characterized Sox9 target, which validates this strategy, whereas retina represents a novel Sox9 function. Analysis of mutant phenotypes confirmed that Sox9 helps regulate the number of Muller glia and photoreceptor cells and helps organize the neural retina. These roles in eye development were previously unrecognized and reinforce the multiple functions that Sox9 plays in vertebrate development. (C) 2009 Elsevier Inc. All rights reserved.
引用
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页码:1 / 15
页数:15
相关论文
共 102 条
[1]   Essential role of Sox9 in the pathway that controls formation of cardiac valves and septa [J].
Akiyama, H ;
Chaboissier, MC ;
Behringer, RR ;
Rowitch, DH ;
Schedl, A ;
Epstein, JA ;
de Crombrugghe, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (17) :6502-6507
[2]   Zebrafish hox clusters and vertebrate genome evolution [J].
Amores, A ;
Force, A ;
Yan, YL ;
Joly, L ;
Amemiya, C ;
Fritz, A ;
Ho, RK ;
Langeland, J ;
Prince, V ;
Wang, YL ;
Westerfield, M ;
Ekker, M ;
Postlethwait, JH .
SCIENCE, 1998, 282 (5394) :1711-1714
[3]   Targeted mutagenesis of the endogenous mouse Mis gene promoter:: In vivo definition of genetic pathways of vertebrate sexual development [J].
Arango, NA ;
Lovell-Badge, R ;
Behringer, RR .
CELL, 1999, 99 (04) :409-419
[4]   Sox9 is required for invagination of the otic placode in mice [J].
Barrionuevo, Francisco ;
Naumann, Angela ;
Bagheri-Fam, Stefan ;
Speth, Volker ;
Taketo, Makoto M. ;
Scherer, Gerd ;
Neubueser, Annette .
DEVELOPMENTAL BIOLOGY, 2008, 317 (01) :213-224
[5]   Sox9 is required for notochord maintenance in mice [J].
Barrionuevo, Francisco ;
Taketo, Makoto M. ;
Scherer, Gerd ;
Kispert, Andreas .
DEVELOPMENTAL BIOLOGY, 2006, 295 (01) :128-140
[6]   Sox9 regulates cell proliferation and is required for Paneth cell differentiation in the intestinal epithelium [J].
Bastide, Pauline ;
Darido, Charbel ;
Pannequiri, Julie ;
Kist, Ralf ;
Robine, Sylvie ;
Marty-Double, Christiane ;
Bibeau, Frederic ;
Scherer, Gerd ;
Joubert, Dominique ;
Hollande, Frederic ;
Blache, Philippe ;
Jay, Philippe .
JOURNAL OF CELL BIOLOGY, 2007, 178 (04) :635-648
[7]   SOX9 directly regulates the type-II collagen gene [J].
Bell, DM ;
Leung, KKH ;
Wheatley, SC ;
Ng, LJ ;
Zhou, S ;
Ling, KW ;
Sham, MH ;
Koopman, P ;
Tam, PPL ;
Cheah, KSE .
NATURE GENETICS, 1997, 16 (02) :174-178
[8]   Notch-Delta signaling is required for spatial patterning and Muller glia differentiation in the zebrafish retina [J].
Bernardos, RL ;
Lentz, SI ;
Wolfe, MS ;
Raymond, PA .
DEVELOPMENTAL BIOLOGY, 2005, 278 (02) :381-395
[9]   Haploinsufficiency of Sox9 results in defective cartilage primordia and premature skeletal mineralization [J].
Bi, WM ;
Huang, WD ;
Whitworth, DJ ;
Deng, JM ;
Zhang, ZP ;
Behringer, RR ;
de Crombrugghe, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (12) :6698-6703
[10]   Sox9 is required for cartilage formation [J].
Bi, WM ;
Deng, JM ;
Zhang, ZP ;
Behringer, RR ;
de Crombrugghe, B .
NATURE GENETICS, 1999, 22 (01) :85-89