Absence of mutations associated with sulfa resistance in Pneumocystis carinii dihydropteroate synthase gene from non-human primates

被引：8
作者：
Demanche, C
Guillot, J
Berthelemy, M
Petit, T
Roux, P
Wakefield, AE
机构：
[1] Ecole Natl Vet Alfort, UMR 956 INRA, AFSSA ENVA, F-94704 Maisons Alfort, France
[2] Univ Paris 06, Lab Parasitol Mycol, F-75012 Paris, France
[3] Univ Oxford, Inst Mol Med, Mol Infect Dis Grp, Oxford, England
来源：
MEDICAL MYCOLOGY | 2002年 / 40卷 / 03期
关键词：
dihydropteroate synthase; mutation; Pneumocystis carinii; primates;
D O I：
10.1080/714031111
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
The dihydropteroate synthase (DHPS) gene front Pneumocystis. carinii. isolated from non-human primates was amplified using a polymerase chain reaction (PCR) and sequenced to analyse point mutations associated with sulfa resistance. P. carinii DHPS gene amplification was obtained from eight lung samples from five New World primate species and one Old World primate species. None of the animals had been exposed to sulfa drugs and only the wild-type P. carinii DHPS sequence at codons 55 and 57 was observed. These data support the hypothesis that high rates of DHPS mutants in P. carinii f. sp. hominis have arisen with increased use of sulfa drugs for P. carinii pneumonia prophylaxis.
引用
收藏
页码：315 / 318
页数：4
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