Diabetes Induced Renal Urea Transport Alterations Assessed With 3D Hyperpolarized 13C, 15N-Urea

被引：24
作者：
Bertelsen, Lotte B. [1 ]
Nielsen, Per M. [1 ]
Qi, Haiyun [1 ]
Norlinger, Thomas S. [1 ]
Zhang, Xiaolu [1 ]
Stodkilde-Jorgensen, Hans [1 ]
Laustsen, Christoffer [1 ]
机构：
[1] Aarhus Univ, MR Res Ctr, Dept Clin Med, Aarhus, Denmark
来源：
MAGNETIC RESONANCE IN MEDICINE | 2017年 / 77卷 / 04期
关键词：
MRI; Type; 1; diabetes; kidney; renal function; hyperpolarization; RAT-KIDNEY; MELLITUS; METABOLISM; PROTEINS; DISEASE;
D O I：
10.1002/mrm.26256
中图分类号：
R8 [特种医学]; R445 [影像诊断学];
学科分类号：
1002 ; 100207 ; 1009 ;
摘要：
Purpose: In the current study, we investigated hyperpolarized urea as a possible imaging biomarker of the renal function by means of the intrarenal osmolality gradient. Methods: Hyperpolarized three-dimensional balanced steady state C-13 MRI experiments alongside kidney function parameters and quantitative polymerase chain reaction measurements was performed on two groups of rats, a streptozotocin type 1 diabetic group and a healthy control group. Results: A significant decline in intrarenal steepness of the urea gradient was found after 4 weeks of untreated insulinopenic diabetes in agreement with an increased urea transport transcription. Conclusion: MRI and hyperpolarized [C-13, N-15] urea can monitor the changes in the corticomedullary urea concentration gradients in diabetic and healthy control rats. Magn Reson Med 77: 1650-1655, 2017. (C) 2016 International Society for Magnetic Resonance in Medicine
引用
收藏
页码：1650 / 1655
页数：6
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