Emerging techniques in the isolation and characterization of extracellular vesicles and their roles in cancer diagnostics and prognostics

被引：88

作者：

Sunkara, Vijaya ^{[1
]}

Woo, Hyun-Kyung ^{[1
]}

Cho, Yoon-Kyoung ^{[1
,2
]}

机构：

[1] UNIST, Sch Life Sci, Dept Biomed Engn, Ulsan 689798, South Korea

[2] IBS, Ctr Soft & Living Matter, UNIST Gil 50, Ulsan 689798, South Korea

来源：

ANALYST | 2016年 / 141卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

NANOPARTICLE TRACKING ANALYSIS; CELL-DERIVED EXOSOMES; BIOMARKER; MICROPARTICLES; MICROVESICLES; PURIFICATION; MICRORNAS; QUANTIFICATION; SENSITIVITY; BIOGENESIS;

D O I：

10.1039/c5an01775k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Extracellular vesicles (EVs) are cell-derived nanovesicles, present in almost all types of body fluids, which play an important role in intercellular communication and are involved in the transport of biological signals for regulating diverse cellular functions. Due to the increasing clinical interest in the role of EVs in tumor promotion, various techniques for their isolation, detection, and characterization are being developed. In this review, we present an overview of the current EV isolation and characterization methods in addition to their applications and limitations. Furthermore, EVs as the potential emerging biomarkers in cancer management and their clinical implementation are briefly discussed.

引用

页码：371 / 381

页数：11

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