Radionecrosis and cellular changes in small volume stereotactic brain radiosurgery in a porcine model

被引：13

作者：

Zaer, Hamed ^{[1
,2
]}

Glud, Andreas Norgaard ^{[1
,2
]}

Schneider, Bret M. ^{[3
,5
,6
]}

Lukacova, Slavka ^{[2
,7
]}

Hansen, Kim Vang ^{[8
,9
]}

Adler, John R. ^{[3
,5
]}

Hoyer, Morten ^{[4
]}

Jensen, Morten Bjorn ^{[2
,7
]}

Hansen, Rune ^{[2
,7
]}

Hoffmann, Lone ^{[2
,7
]}

Worm, Esben Schjodt ^{[2
,7
]}

Sorensen, Jens Chr. Hedemann ^{[1
,2
]}

Orlowski, Dariusz ^{[1
,2
]}

机构：

[1] Aarhus Univ Hosp, Ctr Expt Neurosci CENSE, Dept Neurosur, Palle Juul Jensens Blvd 165,Plan 1,J118-125, DK-8200 Aarhus N, Denmark

[2] Aarhus Univ, Dept Clin Med, Aarhus, Denmark

[3] Zap Surg Syst Inc, San Carlos, CA USA

[4] Aarhus Univ Hosp, Danish Ctr Particle Therapy, Aarhus, Denmark

[5] Stanford Univ, Dept Neurosurg, Sch Med, Stanford, CA 94305 USA

[6] Stanford Univ, Dept Psychiat & Behav Sci, Sch Med, Stanford, CA 94305 USA

[7] Aarhus Univ Hosp, Dept Oncol & Radiat Therapy, Aarhus, Denmark

[8] Aarhus Univ & Hosp, Inst Clin Med, Dept Nucl Med, Aarhus, Denmark

[9] Aarhus Univ & Hosp, Inst Clin Med, PET Ctr, Aarhus, Denmark

来源：

SCIENTIFIC REPORTS | 2020年 / 10卷 / 01期

关键词：

GAMMA-KNIFE SURGERY; CEREBRAL RADIATION NECROSIS; WHITE-MATTER; RAT-BRAIN; IRRADIATION; CELLS; CNS; RADIOTHERAPY; ACTIVATION; TOLERANCE;

D O I：

10.1038/s41598-020-72876-w

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Stereotactic radiosurgery (SRS) has proven an effective tool for the treatment of brain tumors, arteriovenous malformation, and functional conditions. However, radiation-induced therapeutic effect in viable cells in functional SRS is also suggested. Evaluation of the proposed modulatory effect of irradiation on neuronal activity without causing cellular death requires the knowledge of radiation dose tolerance at very small tissue volume. Therefore, we aimed to establish a porcine model to study the effects of ultra-high radiosurgical doses in small volumes of the brain. Five minipigs received focal stereotactic radiosurgery with single large doses of 40-100 Gy to 5-7.5 mm fields in the left primary motor cortex and the right subcortical white matter, and one animal remained as unirradiated control. The animals were followed-up with serial MRI, PET scans, and histology 6 months post-radiation. We observed a dose-dependent relation of the histological and MRI changes at 6 months post-radiation. The necrotic lesions were seen in the grey matter at 100 Gy and in white matter at 60 Gy. Furthermore, small volume radiosurgery at different dose levels induced vascular, as well as neuronal cell changes and glial cell remodeling.

引用

页数：12

共 59 条

[1] Caspase-3 activation in astrocytes following postnatal excitotoxic damage correlates with cytoskeletal remodeling but not with cell death or proliferation [J].