Purification, structural characterization and antiproliferative properties of chondroitin sulfate/dermatan sulfate from tunisian fish skins

被引：30

作者：

Krichen, Fatma ^{[1
]}

Volpi, Nicola ^{[2
]}

Sila, Assaad ^{[1
,3
]}

Maccari, Francesca ^{[2
]}

Mantovani, Veronica ^{[2
]}

Galeotti, Fabio ^{[2
]}

Ellouz-Chaabouni, Semia ^{[1
]}

Bougatef, Ali ^{[1
,4
]}

机构：

[1] Univ Sfax, Lab Ameliorat Plantes & Valorisat Agrores, Sfax 3038, Tunisia

[2] Univ Modena & Reggio Emilia, Dept Life Sci, Modena, Italy

[3] Univ Lille 1, Equipe ProBioGEM, Inst Reg Rech Agroalimentaire & Biotechnol Charle, EA1026, F-59655 Villeneuve Dascq, France

[4] Univ Sfax, Higher Inst Biotechnol, BP 1175, Sfax 3038, Tunisia

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2017年 / 95卷

关键词：

Glycosaminoglycans; Chondroitin sulfate; Dermatan sulfate; Antiproliferative activity; Fish skins; HEPARIN-LIKE GLYCOSAMINOGLYCAN; DERMATAN SULFATE; CAPILLARY-ELECTROPHORESIS; COLORIMETRIC ASSAY; ANTITUMOR-ACTIVITY; GLUCOSAMINE; POLYSACCHARIDES; ANTICOAGULANT; ACTIVATION; EXTRACTION;

D O I：

10.1016/j.ijbiomac.2016.10.108

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chondroitin sulfate/dermatan sulfate GAGs were extracted and purified from the skins of grey triggerfish (GTSG) and smooth hound (SHSG). The disaccharide composition produced by chondroitinase ABC treatment showed the presence of nonsulfated disaccharide, monosulfated disaccharides Delta Di6S and Delta D14S, and disulfated disaccharides in different percentages. In particular, the nonsulfated disaccharide Delta DI0S of GTSG and SHSG were 3.5% and 5.5%, respectively, while monosulfated disaccharides Delta Di6Sand Delta Di4S were evaluated to be 18.2%, 59% and 14.6%, 47.0%, respectively. Capillary elecrophoresis analysis of GTSG and SHSG contained 99.2% and 95.4% of chondroitin sulfate/dermatan sulfate, respectively. PAGE analysis showed a GTSG and SHSG having molecular masses with average values of 41.72 KDa and 23.8 KDa, respectively. HCT116 cell proliferation was inhibited (p <0.05) by 70.6% and 72.65% at 200 mu g/mL of GTSG and SHSG respectively. Both GTSG and SHSG demonstrated promising antiproliferative potential, which may be used as a novel, effective agent. (C) 2016 Elsevier B.V. All rights

引用

页码：32 / 39

页数：8

共 54 条

[1] Fucoidan induces apoptosis of human HS-Sultan cells accompanied by activation of caspase-3 and down-regulation of ERK pathways [J].