Molecular cloning and expression analysis of pig CD138

被引：0

作者：

Bae, Joonbeom ^{[1
]}

Jeong, Seonah ^{[1
]}

Lee, Ju Yeon ^{[1
]}

Lee, Hyun-Jeong ^{[2
]}

Choi, Bong-Hwan ^{[2
]}

Kim, Ji-Eun ^{[3
]}

Choi, Inho ^{[3
]}

Chun, Taehoon ^{[1
]}

机构：

[1] Korea Univ, Sch Life Sci & Biotechnol, Div Biotechnol, Seoul 136701, South Korea

[2] Rural Dev Adm, Natl Inst Anim Sci, Div Anim Genom & Bioinformat, Suwon 14313, South Korea

[3] Yeungnam Univ, Sch Biotechnol, Kyongsan 712749, South Korea

来源：

RESEARCH IN VETERINARY SCIENCE | 2013年 / 95卷 / 03期

关键词：

cDNA cloning; CD138; Expression; Pig; Syndecan; HEPARAN-SULFATE PROTEOGLYCANS; SYNDECAN-1; DISEASE; GROWTH; FAMILY;

D O I：

10.1016/j.rvsc.2013.09.010

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

CD138 (syndecan-1) interacts with various components of the extracellular matrix and associates with the actin cytoskeleton. In this study, we cloned pig CD138 cDNA and determined its complete cDNA sequence. Pig CD138 cDNA contained an open reading frame (930 bp) encoding 309 amino acids with five well conserved putative glycosaminoglycan attachment sites, a putative cleavage site for matrix metalloproteinases, and conserved motifs involved in signal transduction among mammalian species. Pig CD138 mRNA was detected in various tissues, including lymphoid and non-lymphoid organs, indicating the multicellular functions of CD138 in pigs. Western blot and flow cytometry analyses detected an approximate 35 kDa pig CD138 protein expressed on the cell surface. Further immunohistochemistry analysis revealed that CD138 expression was mainly observed in submucosa and lamina propria of the pig small intestine. Further study will be necessary to define the functional importance of CD138 during specific infectious diseases in pigs. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：1021 / 1025

页数：5

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