MONOLITHIC COLUMNS FOR SEPARATION OF LOW-MOLECULAR COMPOUNDS

被引:0
|
作者
Stankova, Magda [1 ]
Jandera, Pavel [1 ]
Urban, Jiri [1 ]
Skerikova, Veronika [1 ]
机构
[1] Univ Pardubice, Fac Chem Technol, Dept Analyt Chem, Pardubice 53210, Czech Republic
来源
CECE 2012: 9TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS | 2012年
关键词
Organic polymer monoliths; Efficiency; Low-molecular compounds;
D O I
暂无
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Crosslinking monomers with various lengths of methylene or oxyethylene chains between two methacrylate units were applied for preparation of polymethacrylate monolithic columns suitable for isocratic separation of low-molecular compounds, using lauryl methacrylate as the functional monomer for reversed-phase chromatography. The crosslinking monomers included ethylene-, tetramethylene-, hexamethylene-, dioxyethylene-, trioxyethylene-, and tetraoxyethylene dimethacrylates. The replacement of generally used ethylene dimethacrylate as a crosslinking monomer by monomers with longer methylene and oxyethylene chain led to significant increase in the column efficiency. For example, a column prepared with tetraoxyethylene dimethacrylate shows the column efficiency of 71 000 theoretical plates per meter and offers fast isocratic separation of six low-molecular alkylbenzenes in 2 minutes. This confirms the significant effect of the length of the oxyethylene chain in the crosslinking monomer on the efficiency of polymethacrylate capillary columns.
引用
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页码:310 / 313
页数:4
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