MONOLITHIC COLUMNS FOR SEPARATION OF LOW-MOLECULAR COMPOUNDS

被引：0

作者：

Stankova, Magda ^{[1
]}

Jandera, Pavel ^{[1
]}

Urban, Jiri ^{[1
]}

Skerikova, Veronika ^{[1
]}

机构：

[1] Univ Pardubice, Fac Chem Technol, Dept Analyt Chem, Pardubice 53210, Czech Republic

来源：

CECE 2012: 9TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS | 2012年

关键词：

Organic polymer monoliths; Efficiency; Low-molecular compounds;

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Crosslinking monomers with various lengths of methylene or oxyethylene chains between two methacrylate units were applied for preparation of polymethacrylate monolithic columns suitable for isocratic separation of low-molecular compounds, using lauryl methacrylate as the functional monomer for reversed-phase chromatography. The crosslinking monomers included ethylene-, tetramethylene-, hexamethylene-, dioxyethylene-, trioxyethylene-, and tetraoxyethylene dimethacrylates. The replacement of generally used ethylene dimethacrylate as a crosslinking monomer by monomers with longer methylene and oxyethylene chain led to significant increase in the column efficiency. For example, a column prepared with tetraoxyethylene dimethacrylate shows the column efficiency of 71 000 theoretical plates per meter and offers fast isocratic separation of six low-molecular alkylbenzenes in 2 minutes. This confirms the significant effect of the length of the oxyethylene chain in the crosslinking monomer on the efficiency of polymethacrylate capillary columns.

引用

页码：310 / 313

页数：4