Synthesis of a molecularly imprinted polymer for the selective solid-phase extraction of chloramphenicol from honey

被引：89

作者：

Schirmer, Christina ^{[1
]}

Meisel, Hans ^{[1
]}

机构：

[1] BfEL Locat Kiel, Fed Res Ctr Nutr & Food, Inst Dairy Chem & Technol, D-24103 Kiel, Germany

来源：

JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1132卷 / 1-2期

关键词：

molecular imprinting; solid-phase extraction; chloramphenicol; honey samples;

D O I：

10.1016/j.chroma.2006.09.032

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Solid-phase extraction (SPE) with a molecularly imprinted polymer (MIP) as sorbent has been investigated for the clean-up of the broad-spectrum bacteriostatic antibiotic chloramphenicol (CAP) in honey samples. The MIP was prepared by using methacrylic acid (MAA) as functional monomer, ethylene glycol dimethacrylate (EDMA) as cross-linker, chloroform as porogen and CAP as template molecule. The binding behaviour of the template CAP on the MIP was evaluated by high-performance liquid chromatography, and then the MIP was applied as a sorbent in SPE to selectively extract CAP from honey. It was shown that recoveries of nearly 100% of a CAP standard solution and up to 94% from spiked honey samples could be obtained after SPE. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：325 / 328

页数：4

共 11 条

[1]

*DIN, 1994, 32645 DIN

[2]

GROTEWAHL D, 2006, LEBENSMITTELCHEMIE, P35

[3] Optical detection of chloramphenicol using molecularly imprinted polymers [J].

Levi, R ;

McNiven, S ;

Piletsky, SA ;

Cheong, SH ;

Yano, K ;

Karube, I .

ANALYTICAL CHEMISTRY, 1997, 69 (11) :2017-2021

[4] Molecularly imprinted polymers for on-line clean up and preconcentration of chloramphenicol prior to its voltammetric determination [J].

Mena, ML ;

Agüí, L ;

Martinez-Ruiz, P ;

Yáñez-Sedeño, P ;

Reviejo, AJ ;

Pingarrón, JM .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2003, 376 (01) :18-25

[5] Effect of solvents on the selectivity of terbutylazine imprinted polymer sorbents used in solid-phase extraction [J].

Pap, T ;

Horváth, V ;

Tolokán, A ;

Horvai, G ;

Sellergren, B .

JOURNAL OF CHROMATOGRAPHY A, 2002, 973 (1-2) :1-12

[6]

RAEZKE KP, 2006, COMMUNICATION

[7] Imprinted chiral stationary phases in high-performance liquid chromatography [J].

Sellergren, B .

JOURNAL OF CHROMATOGRAPHY A, 2001, 906 (1-2) :227-252

[8] DIRECT DRUG DETERMINATION BY SELECTIVE SAMPLE ENRICHMENT ON AN IMPRINTED POLYMER [J].

SELLERGREN, B .

ANALYTICAL CHEMISTRY, 1994, 66 (09) :1578-1582

[9]

Sellergren B., 2001, MOL IMPRINTED POLYM, V23

[10] Fluorescent competitive flow-through assay for chloramphenicol using molecularly imprinted polymers [J].

Suárez-Rodríguez, JL ;

Díaz-García, ME .

BIOSENSORS & BIOELECTRONICS, 2001, 16 (9-12) :955-961

← 1 2 →