Transdermal Delivery of Functional Collagen Via Polyvinylpyrrolidone Microneedles

被引：29

作者：

Sun, Wenchao ^{[1
,2
]}

Inayathullah, Mohammed ^{[1
,2
]}

Manoukian, Martin A. C. ^{[1
,3
]}

Malkovskiy, Andrey V. ^{[1
]}

Manickam, Sathish ^{[1
]}

Marinkovich, M. Peter ^{[3
,4
]}

Lane, Alfred T. ^{[3
]}

Tayebi, Lobat ^{[1
,5
]}

Seifalian, Alexander M. ^{[6
]}

Rajadas, Jayakumar ^{[1
,2
]}

机构：

[1] Stanford Univ, Sch Med, Biomat & Adv Drug Delivery Lab, Stanford, CA 94304 USA

[2] Stanford Univ, Sch Med, Cardiovasc Inst, Cardiovasc Pharmacol Div, Stanford, CA 94305 USA

[3] Stanford Univ, Sch Med, Dept Dermatol, Stanford, CA 94305 USA

[4] Palo Alto VA Med Ctr, Div Dermatol, Palo Alto, CA 94304 USA

[5] Marquette Univ, Sch Dent, Dept Dev Sci, Milwaukee, WI 53201 USA

[6] UCL, Div Surg & Intervent Sci, London, England

来源：

ANNALS OF BIOMEDICAL ENGINEERING | 2015年 / 43卷 / 12期

基金：

美国国家卫生研究院;

关键词：

Collagen; Microneedles; Biomaterials; Transdermal delivery; PIG-EAR SKIN; VACCINE DELIVERY; DRUG-DELIVERY; I COLLAGEN; PROTEIN; PENETRATION; SYSTEM; CELLS; EYE;

D O I：

10.1007/s10439-015-1353-0

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Collagen makes up a large proportion of the human body, particularly the skin. As the body ages, collagen content decreases, resulting in wrinkled skin and decreased wound healing capabilities. This paper presents a method of delivering type I collagen into porcine and human skin utilizing a polyvinylpyrrolidone microneedle delivery system. The microneedle patches were made with concentrations of 1, 2, 4, and 8% type I collagen (w/w). Microneedle structures and the distribution of collagen were characterized using scanning electron microscopy and confocal microscopy. Patches were then applied on the porcine and human skin, and their effectiveness was examined using fluorescence microscopy. The results illustrate that this microneedle delivery system is effective in delivering collagen I into the epidermis and dermis of porcine and human skin. Since the technique presented in this paper is quick, safe, effective and easy, it can be considered as a new collagen delivery method for cosmetic and therapeutic applications.

引用

页码：2978 / 2990

页数：13