Degradation of fluorobenzene by Rhizobiales strain F11 via ortho cleavage of 4-fluorocatechol and catechol

被引：42
作者：
Carvalho, Maria F.
Ferreira, Maria Isabel M.
Moreira, Irina S.
Castro, Paula M. L.
Janssen, Dick B.
机构：
[1] Univ Catolica Portuguesa, Escola Super Biotecnol, P-4200072 Porto, Portugal
[2] Univ Groningen, Groningen Biomol Sci & Biotechnol Inst, Dept Biochem, NL-9747 AG Groningen, Netherlands
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2006年 / 72卷 / 11期
关键词：
D O I：
10.1128/AEM.01162-06
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The aerobic metabolism of fluorobenzene by Rhizobiales sp. strain F11 was investigated. Liquid chromatography-mass spectrometry analysis showed that 4-fluorocatechol and catechol were formed as intermediates during fluorobenzene degradation by cell suspensions. Both these compounds, unlike 3-fluorocatechol, supported growth and oxygen uptake. Cells grown on fluorobenzene contained enzymes for the ortho pathway but not for meta ring cleavage of catechols. The results suggest that fluorobenzene is predominantly degraded via 4-fluorocatechol with subsequent ortho cleavage and also partially via catechol.
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页码：7413 / 7417
页数：5
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